Coustau C, Yoshino T P
Centre de Biologie et d'Ecologie, UMR 5555 du CNRS, Universit¿e de Perpignan, 52 Ave de Villeneuve, Perpignan cedex, 66 860, France.
Exp Parasitol. 2000 Jan;94(1):62-6. doi: 10.1006/expr.1999.4462.
In vitro cultivation of parasitic helminths, including the digenetic trematodes, has long been a valuable tool in medical and veterinary parasitology, permitting and/or facilitating the development of diagnostic reagents, chemotherapeutic agents, and vaccines and providing insights into naturally complex host-parasite interactions. In vitro cultivation of the intramolluscan stages of trematodes has been particularly challenging, given the ontogenic complexities involved in the production of multiple larval generations from germinal tissues through an asexual "budding" process. Recently, however, advanced larval development has been achieved by incorporating the Biomphalaria glabrata embryonic (Bge) cell line into cocultivation systems. Most notably, the entire intramolluscan cycle (from miracidium to cercaria) has been completed for the human blood fluke Schistosoma mansoni, while significant primary sporocyst development has been attained for several other digeneans including S. japonicum and Fascioloides magna. Here we review recent advances in the cultivation of several larval trematode species and discuss the potential use of this culture system for addressing fundamental questions of host-parasite compatibility.
包括复殖吸虫在内的寄生蠕虫的体外培养，长期以来一直是医学和兽医寄生虫学中的一项重要工具，有助于和/或促进诊断试剂、化疗药物和疫苗的开发，并为深入了解天然复杂的宿主 - 寄生虫相互作用提供见解。鉴于从生殖组织通过无性“出芽”过程产生多个幼虫世代所涉及的个体发育复杂性，吸虫在软体动物体内阶段的体外培养一直特别具有挑战性。然而，最近通过将光滑双脐螺胚胎（Bge）细胞系纳入共培养系统，实现了幼虫的进一步发育。最值得注意的是，人类血吸虫曼氏血吸虫已完成了整个在软体动物体内的周期（从毛蚴到尾蚴），而包括日本血吸虫和巨片形吸虫在内的其他几种复殖吸虫也实现了显著的初级孢蚴发育。在此，我们综述了几种幼虫吸虫培养的最新进展，并讨论了该培养系统在解决宿主 - 寄生虫相容性基本问题方面的潜在用途。
