Koizumi N, Inatomi T, Quantock A J, Fullwood N J, Dota A, Kinoshita S
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Cornea. 2000 Jan;19(1):65-71. doi: 10.1097/00003226-200001000-00013.
To examine the viability of using human amniotic membrane as substrate for culturing corneal epithelial cells and transplanting them onto severely injured rabbit eyes.
An ocular-surface injury was created in the right eye of eight rabbits by a lamellar keratectomy extending 5 mm outside the limbus. Next, from the limbal region of the uninjured left eyes of five of these animals, a small biopsy of corneal epithelial cells was taken and cultured on acellular human amniotic membrane. One month later, the invading conjunctiva that covered the corneal surface of all eight injured eyes was surgically removed. Five of the eyes then received grafts of amniotic membrane containing autologous cultured epithelial cells, whereas the other three received grafts of acellular amniotic membrane alone.
A confluent primary culture of limbal corneal epithelial cells was established on acellular human amniotic membrane after 14 days. Cells were partially stratified and fairly well attached to the underlying amniotic membrane, although a fully formed basement membrane was not evident. The three rabbits that received amniotic membrane transplantation alone all had total epithelial defects on the graft in the early postoperative period. Eyes that were grafted with amniotic membrane that contained cultivated epithelial cells, however, were all successfully epithelialized up to 5 days after surgery.
Autologous transplantation of cultivated corneal epithelium is feasible by using acellular amniotic membrane as a carrier.
探讨将人羊膜作为培养角膜上皮细胞并移植到严重损伤兔眼的基质的可行性。
通过在角膜缘外5毫米处进行板层角膜切除术，在8只兔子的右眼造成眼表损伤。接下来，从其中5只动物未受伤的左眼的角膜缘区域取一小片角膜上皮细胞活检组织，在脱细胞人羊膜上进行培养。1个月后，手术切除覆盖所有8只受伤眼睛角膜表面的侵入性结膜。然后，其中5只眼睛接受含有自体培养上皮细胞的羊膜移植，而另外3只眼睛仅接受脱细胞羊膜移植。
14天后在脱细胞人羊膜上建立了角膜缘角膜上皮细胞的汇合原代培养。细胞部分分层，与下面的羊膜附着良好，尽管没有明显的完整基底膜。仅接受羊膜移植的3只兔子在术后早期移植片上均有完全上皮缺损。然而，移植含有培养上皮细胞的羊膜的眼睛在手术后5天内均成功上皮化。
以脱细胞羊膜为载体进行自体培养角膜上皮移植是可行的。
