Saso L, Valentini G, Riccieri V, Spadaro A, Zoppini A, Silvestrini B
Department of Pharmacology of Natural Substances and General Physiology, University of Rome La Sapienza, Italy.
IUBMB Life. 1999 Oct;48(4):385-90. doi: 10.1080/713803529.
A strong increase of the affinity for concanavalin A (Con A) of serum alpha 2-macroglobulin, a non-acute-phase protein, was observed by lectin blotting in patients with Sjögren's syndrome (SS). On the contrary, the total Con A reactivity of serum proteins, measured by enzyme-linked lectin assay, was not augmented in SS, compared with normal donors, probably because positive changes of certain proteins were balanced by negative changes of others, as suggested by lectin blotting analysis. However, a significant increase of total Con A reactivity occurred in subjects with increased serum concentrations of soluble interleukin (IL)-2 receptor, compared with patients with normal concentrations of this marker of disease activity. On the other hand, the same parameter did not appear to be different in patients with normal or increased serum concentrations of IL-6, indicating that this cytokine was not probably responsible for the changes of glycosylation described here.
在干燥综合征（SS）患者中，通过凝集素印迹法观察到血清α2-巨球蛋白（一种非急性期蛋白）对伴刀豆球蛋白A（Con A）的亲和力显著增加。相反，与正常供体相比，通过酶联凝集素测定法测得的血清蛋白的总Con A反应性在SS中并未增强，这可能是因为凝集素印迹分析表明某些蛋白的正向变化被其他蛋白的负向变化所平衡。然而，与该疾病活动标志物浓度正常的患者相比，可溶性白细胞介素（IL）-2受体血清浓度升高的受试者中总Con A反应性显著增加。另一方面，在IL-6血清浓度正常或升高的患者中，相同参数似乎没有差异，这表明该细胞因子可能不是此处所述糖基化变化的原因。
