Chodorowska Grazyna, Czelej Dorota
Chair and Department of Dermatology, Medical University of Lublin, Lublin, Poland.
Ann Univ Mariae Curie Sklodowska Med. 2003;58(1):43-7.
Plasma concentrations of 8 proteins, including cytokines: interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-alpha), receptors: soluble IL-2 receptor (slL-2R), p55 soluble TNF receptor (p55 sTNF-R) and acute phase proteins: alpha-2 macroglobulin (alpha-2 MG), C-reactive protein (CRP) were examined in 14 patients with drug-induced hyperergic vasculitis. The activity of selected proteins were measured using the immunoenzymatic ELISA method: a) in the acute stage of disease before treatment was administered, and b) after clearing of skin lesions, after treatment. In the acute stage of disease highly elevated concentrations of the examined proteins (p<0.001) in comparison to the control were found. After clearing of clinical symptoms the concentrations of IL-6 and alpha-2 MG were not significantly different from the control values. But despite deep decrease, plasma levels of remaining six proteins were still highly significant (p<0.001) or significant (p<0.01) when compared to the control. Results of this study indicate that in the course of drug-induced hyperergic vasculitis the systemic inflammatory and immune response is activated and elevated concentrations of the examined proteins are present in peripheral blood despite clearing of the clinical symptoms of the disease.
检测了14例药物性超敏性血管炎患者血浆中8种蛋白质的浓度，包括细胞因子：白细胞介素-2（IL-2）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α），受体：可溶性IL-2受体（slL-2R）、p55可溶性TNF受体（p55 sTNF-R）以及急性期蛋白：α-2巨球蛋白（α-2 MG）、C反应蛋白（CRP）。采用免疫酶联ELISA法检测所选蛋白质的活性：a）在疾病急性期治疗前，b）治疗后皮肤病变消退后。在疾病急性期，与对照组相比，所检测蛋白质的浓度显著升高（p<0.001）。临床症状消退后，IL-6和α-2 MG的浓度与对照值无显著差异。但尽管大幅下降，与对照组相比，其余六种蛋白质的血浆水平仍高度显著（p<0.001）或显著（p<0.01）。本研究结果表明，在药物性超敏性血管炎病程中，全身炎症和免疫反应被激活，尽管疾病的临床症状已消退，但外周血中所检测蛋白质的浓度仍升高。
