Burchardt T, Burchardt M, Chen M W, Buttyan R, de la Taille A, Shabsigh A, Shabsigh R
Department of Urology, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
IUBMB Life. 1999 Oct;48(4):405-8. doi: 10.1080/713803545.
Currently, at least five different mRNA species encoding vascular endothelial growth factor-A (VEGF-A) have been characterized. These variants result from alternative splicing of the VEGF-A transcript and encode human isoforms of VEGF protein of 121, 145, 165, 189, and 206 amino acids. In the rat, a similar profile of VEGF-A splice variants has been described, each encoding one fewer amino acid than the human species. Studies of mammalian tissues have shown that these mRNA isoforms vary in abundance. Whereas VEGF 188/189, 164/165, and 120/121 (rat/human, respectively) are the predominant forms expressed in most tissues and cells examined, VEGF 144/145 and 205/206 are rare variants. Previously, VEGF 144/145 had been detected only in placental and uterine tissues and endometrial carcinoma cell lines, whereas VEGF 205/206 was detected only in fetal liver and placenta. Using an RT-PCR technique, cDNA cloning, and sequencing, we have detected and confirmed the presence of mRNA encoding VEGF 144/145 and 205/206 in both adult rat lung and penis. Therefore, these low-abundance VEGF splice variants have a more diverse expression pattern than originally predicted.
目前，至少有五种不同的编码血管内皮生长因子-A（VEGF-A）的mRNA种类已得到鉴定。这些变体是VEGF-A转录本选择性剪接的结果，编码121、145、165、189和206个氨基酸的VEGF蛋白的人类异构体。在大鼠中，已描述了类似的VEGF-A剪接变体谱，每种变体编码的氨基酸比人类异构体少一个。对哺乳动物组织的研究表明，这些mRNA异构体在丰度上有所不同。虽然VEGF 188/189、164/165和120/121（分别为大鼠/人类）是在大多数检查的组织和细胞中表达的主要形式，但VEGF 144/145和205/206是罕见的变体。以前，仅在胎盘和子宫组织以及子宫内膜癌细胞系中检测到VEGF 144/145，而仅在胎儿肝脏和胎盘中检测到VEGF 205/206。使用逆转录聚合酶链反应（RT-PCR）技术、cDNA克隆和测序，我们已经在成年大鼠肺和阴茎中检测并确认了编码VEGF 144/145和205/206的mRNA的存在。因此，这些低丰度的VEGF剪接变体具有比最初预测的更多样化的表达模式。
