从古代和现代木材中扩增橡树DNA。

Amplification of oak DNA from ancient and modern wood.

作者信息

Dumolin-Lapègue S, Pemonge M H, Gielly L, Taberlet P, Petit R J

机构信息

Laboratoire de Génétique et Amélioration des Abres Forestiers, INRA, BP 45-F-33611 Gazinet Cedex, France.

出版信息

Mol Ecol. 1999 Dec;8(12):2137-40. doi: 10.1046/j.1365-294x.1999.00788.x.

DOI:10.1046/j.1365-294x.1999.00788.x

PMID:10632865

Abstract

A polymorphic noncoding region of chloroplast DNA (cpDNA) was successfully amplified by the polymerase chain reaction (PCR) from various oak wood samples, including recent and more ancient (about 600-years-old) samples from different oak species. Adaptation of DNA isolation and amplification protocols was necessary to obtain this result. Polymorphisms useful to distinguish species or geographical origin of these samples could be scored through sequencing. These polymorphisms include one substitution and two microsatellite-type polymorphisms, due to a variable number of A/T repeats. Identical results were obtained independently in two separate laboratories.

摘要

通过聚合酶链反应（PCR）成功地从各种橡木样本中扩增出叶绿体DNA（cpDNA）的一个多态性非编码区域，这些样本包括来自不同橡木种类的近期样本以及更古老（约600年历史）的样本。为获得这一结果，有必要对DNA分离和扩增方案进行调整。通过测序可以确定有助于区分这些样本的物种或地理来源的多态性。这些多态性包括一个单碱基替换和两个微卫星型多态性，这是由于A/T重复序列数量可变所致。在两个独立的实验室中分别独立获得了相同的结果。