来自古代木材遗迹的经鉴定的DNA。
Authenticated DNA from ancient wood remains.
作者信息
Liepelt Sascha, Sperisen Christoph, Deguilloux Marie-France, Petit Remy J, Kissling Roy, Spencer Matthew, de Beaulieu Jacques-Louis, Taberlet Pierre, Gielly Ludovic, Ziegenhagen Birgit
机构信息
Philipps-University Marburg, Faculty of Biology Nature Conservation Division, Karl-von-Frisch-Str 2, Marburg, Germany.
出版信息
Ann Bot. 2006 Nov;98(5):1107-11. doi: 10.1093/aob/mcl188. Epub 2006 Sep 20.
BACKGROUND
The reconstruction of biological processes and human activities during the last glacial cycle relies mainly on data from biological remains. Highly abundant tissues, such as wood, are candidates for a genetic analysis of past populations. While well-authenticated DNA has now been recovered from various fossil remains, the final 'proof' is still missing for wood, despite some promising studies.
SCOPE
The goal of this study was to determine if ancient wood can be analysed routinely in studies of archaeology and palaeogenetics. An experiment was designed which included blind testing, independent replicates, extensive contamination controls and rigorous statistical tests. Ten samples of ancient wood from major European forest tree genera were analysed with plastid DNA markers.
CONCLUSIONS
Authentic DNA was retrieved from wood samples up to 1,000 years of age. A new tool for real-time vegetation history and archaeology is ready to use.
背景
末次冰期循环期间生物过程和人类活动的重建主要依赖于生物遗迹的数据。诸如木材等含量丰富的组织是过去种群基因分析的候选对象。尽管现在已经从各种化石遗迹中成功提取出了经过充分验证的DNA，但尽管有一些前景看好的研究，木材的最终“证据”仍未找到。
范围
本研究的目的是确定在考古学和古遗传学研究中是否可以常规分析古代木材。设计了一项实验，包括盲测、独立重复、广泛的污染控制和严格的统计测试。使用质体DNA标记对来自欧洲主要森林树种的10个古代木材样本进行了分析。
结论
从年龄达1000年的木材样本中提取到了可信的DNA。一种用于实时植被历史和考古学的新工具已可投入使用。
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