Cho J K, Kwon H S, Joo K H, Lee J S, Cho S W
Department of Parasitology, College of Medicine, Korea University, Seoul, Korea.
Korean J Parasitol. 1999 Dec;37(4):285-7. doi: 10.3347/kjp.1999.37.4.285.
Changes in the expression level of splenocyte IFN-gamma mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of IFN-gamma had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with P. westermani, the levels of IFN-gamma mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.
采用竞争性逆转录-聚合酶链反应（RT-PCR），随后进行Southern印迹法，分析感染卫氏并殖吸虫的斯普拉格-道利（SD）大鼠脾细胞干扰素-γ（IFN-γ）mRNA表达水平的变化。模板RNA从感染20个卫氏并殖吸虫囊蚴的大鼠脾细胞中提取。竞争性RT-PCR产物进行Southern印迹和增强化学发光（ECL），并用密度计进行分析。与未感染对照大鼠脾细胞（值为1）相比，感染后1周（PI）时IFN-γ的mRNA表达水平变为0.747，感染后2周时为0.00175，感染后3周时为0.0217，感染后4周时为0.194，然后在感染后5周时变为0.537。感染后7周时该水平恢复到1.25，与未感染大鼠相当。这些结果表明，感染卫氏并殖吸虫时，SD大鼠脾细胞IFN-γ mRNA水平在感染后2周时显著降低超过500倍，并在感染后7周时恢复到正常水平。
