Ringvold A, Anderssen E, Kjønniksen I
The National Hospital Pharmacy, Oslo, Norway.
Invest Ophthalmol Vis Sci. 2000 Jan;41(1):20-3.
To analyze the ascorbate distribution in the anterior eye wall to better understand the functional significance of this compound in the eye.
Ascorbic acid was determined by high-performance liquid chromatography using an LC-10 system (Shimadzu, Kyoto, Japan). Bovine eye samples were used.
The highest ascorbate concentration was observed in the corneal epithelium, with significantly higher values in the central (1.56 mg/g) than in the peripheral (1.39 mg/g) area. The ascorbate content was similar in the corneal stroma (0.22 mg/g), the Descemet's membrane (DM)/endothelium (0.22 mg/g), and the aqueous humor (0.21 mg/ml). By comparison, the sclera (0.15 mg/g) and the conjunctiva (0.11 mg/g) showed lower values, as did the lacrimal gland (0.09 mg/g) and the serum (0.0008 mg/ml).
(1) Peak ascorbate concentration was observed in the central corneal epithelium covering the pupillary area. This is compatible with the idea that the ascorbate may act as an UV filter shielding internal eye structures from radiation damage. (2) The ascorbate concentration in the corneal stroma and DM/endothelium was as high as in the aqueous humor, and it is suggested that the aqueous humor plays a key role in the distribution of ascorbate to the anterior eye wall.
分析抗坏血酸在前眼壁中的分布情况，以更好地理解该化合物在眼中的功能意义。
使用LC - 10系统（日本京都岛津公司）通过高效液相色谱法测定抗坏血酸。使用牛眼样本。
在角膜上皮中观察到最高的抗坏血酸浓度，中央区域（1.56毫克/克）的值显著高于周边区域（1.39毫克/克）。角膜基质（0.22毫克/克）、Descemet膜（DM）/内皮（0.22毫克/克）和房水（0.21毫克/毫升）中的抗坏血酸含量相似。相比之下，巩膜（0.15毫克/克）和结膜（0.11毫克/克）的值较低，泪腺（0.09毫克/克）和血清（0.00...
