Kamper E F, Papaphilis A D, Angelopoulou M K, Kopeikina L T, Siakantaris M P, Pangalis G A, Stavridis J C
Department of Experimental Physiology, School of Medicine, National and Kapodistrian University of Athens, Greece.
Clin Biochem. 1999 Nov;32(8):639-45. doi: 10.1016/s0009-9120(99)00066-1.
The fibrinolytic regulator tetranectin (TN), in association with the circulating intercellular adhesive molecule-1 (cICAM-1) and interleukin -10 (IL-10), may be involved in the metastatic cascade of B-chronic lymphocytic leukemia (B-CLL). Our aim was to investigate the potential usefulness of these molecules as prognostic markers in B-CLL.
Therefore, TN, cICAM-1, and IL-10 were assessed (ELISA) in the serum of 53 B-CLL patients, classified in Binet A, B, and C stages in comparison with those in 45 healthy subjects (HS).
TN was significantly lower in B-CLL patients than in HS (9.63 [8.75-11.51] mg/L, 13.75 [12.56-14.64] ng/mL, respectively, p<10(-5)), being lower (p = 0.05) in B and C stage patients (subgroup B+C) than in A stage ones (subgroup A). cICAM-1 levels were significantly higher in B-CLL patients than in HS (475.86 [355.86-593.79] ng/mL vs. 225.62 [118.49-312.83] ng/mL, respectively, p<10(-5)) with a tendency for higher levels in subgroup B+C than in subgroup A. A significant correlation of cICAM-1 with lactate dehydrogenase (LDH) (r(s) = 0.532, p = 0.049), and a significant increase in cICAM-1 in B-CLL with diffuse bone marrow infiltration (BMI) compared to that in B-CLL with nondiffuse BMI (624.48 [557.24-726.55] ng/mL vs. 480.34 [368.96-590.34] ng/mL, respectively, p = 0.0172) were found. A significant negative correlation between TN and cICAM-1 (r = -0.5017, p = 0.0001) was observed. IL-10 was detected in all B-CLL patients and in no HS (7.37 [5.30-10.55] pg/mL), being higher (p = 0.0153) in C than in A stage patients. A significant correlation of IL-10 with TN and cICAM-1 in subgroup B+C (r(s) = -0.659 [p = 0.014] and r = 0.679 [p = 0.011], respectively) was found.
The abovementioned findings and good performance characteristics of TN and cICAM-1 in B-CLL suggest the potential usefulness of these adhesive/recognition molecules as prognostic markers in B-CLL. The implication of these molecules along with IL-10 in the disease process deserves further study.
纤维蛋白溶解调节剂纤连蛋白（TN）与循环中的细胞间黏附分子-1（cICAM-1）及白细胞介素-10（IL-10）相关，可能参与B细胞慢性淋巴细胞白血病（B-CLL）的转移过程。我们的目的是研究这些分子作为B-CLL预后标志物的潜在用途。
因此，采用酶联免疫吸附测定（ELISA）法检测了53例B-CLL患者血清中的TN、cICAM-1和IL-10，并将患者按照Binet分期分为A、B和C期，同时与45名健康受试者（HS）进行比较。
B-CLL患者血清中的TN显著低于健康受试者（分别为9.63 [8.75 - 11.51] mg/L和13.75 [12.56 - 14.64] ng/mL，p < 10⁻⁵），B期和C期患者（B + C亚组）低于A期患者（A亚组）（p = 0.05）。B-CLL患者血清中的cICAM-1水平显著高于健康受试者（分别为475.86 [355.86 - 593.79] ng/mL和225.62 [118.49 - 312.83] ng/mL，p < 10⁻⁵），B + C亚组的水平有高于A亚组的趋势。cICAM-1与乳酸脱氢酶（LDH）显著相关（rₛ = 0.532，p = 0.049），与无弥漫性骨髓浸润（BMI）的B-CLL患者相比，有弥漫性BMI的B-CLL患者血清中的cICAM-1显著升高（分别为624.48 [557.24 - 726.55] ng/mL和480.34 [368.96 - 590.34] ng/mL，p = 0.0172）。观察到TN与cICAM-1之间存在显著负相关（r = -0.5017，p = 0.0001）。所有B-CLL患者均检测到IL-10，而健康受试者中未检测到（7.37 [5.30 - 10.55] pg/mL），C期患者的IL-10水平高于A期患者（p = 0.0153）。在B + C亚组中发现IL-10与TN和cICAM-1显著相关（分别为rₛ = -0.659 [p = 0.014]和r = 0.679 [p = 0.011]）。
上述研究结果以及TN和cICAM-1在B-CLL中的良好性能特征表明，这些黏附/识别分子作为B-CLL预后标志物具有潜在用途。这些分子与IL-10在疾病过程中的作用值得进一步研究。
