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[透明带部分切割对牛卵母细胞体外受精及随后胚胎体外发育的影响]

[The effect of partial dissection of the zona pellucida on the fertilization of bovine oocytes in vitro and on the subsequent development of the embryos outside the body].

作者信息

Basovskiĭ D N

出版信息

Tsitol Genet. 1999 Sep-Oct;33(5):58-64.

Abstract

Influence of partial zona dissection (PZD) on fertilization and cleavage of cow oocytes and on pre-implantation development of embryos obtained by this method was investigated. Decreased concentration of spermatozoa in less degree influenced on rates of fertilization and cleavage of oocytes with partial zona dissection than on intact eggs. The embryos obtained by method PZD can develop in vitro to blastocyst stage. However, their development is slowed down and presence of dissection in zona pellucida can result in premature hatching such blastocysts.

摘要

研究了部分透明带切割(PZD)对牛卵母细胞受精和卵裂以及通过该方法获得的胚胎植入前发育的影响。精子浓度降低对经部分透明带切割的卵母细胞受精和卵裂率的影响程度小于对完整卵的影响。通过PZD方法获得的胚胎能够在体外发育到囊胚阶段。然而,它们的发育会减慢,透明带中的切割存在可能导致此类囊胚过早孵化。

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