Lacham O, Trounson A, Holden C, Mann J, Sathananthan H
Centre for Early Human Development, Monash University, Monash Medical Centre, Clayton, Victoria, Australia.
Gamete Res. 1989 Jun;23(2):233-43. doi: 10.1002/mrd.1120230210.
Mouse spermatozoa were capacitated and acrosome reacted by incubation for 30 min to 6 h in modified Tyrode's medium (T6) and in cGMP. The fertilization rates of eggs microinjected with a spermatozoon from samples incubated for 30 min, 2 h, and 6 h in T6 and in cGMP were 36%, 34%, 29%, and 43%, respectively. These rates were not correlated to the percentage of acrosome-reacted spermatozoa identified after these treatments. The lack of association could be explained by the selection of an increased proportion of acrosome-reacted spermatozoa actually used for microinjection, particularly in samples incubated for 30 min and 2 h in T6. Both acrosome-intact and acrosome-reacted spermatozoa were identified under the zona in the eggs which failed to fertilize. Fertilized eggs developed at very high rates to the blastocyst stage in vitro.
将小鼠精子在改良的台氏液（T6）和环鸟苷酸（cGMP）中孵育30分钟至6小时，使其获能并发生顶体反应。用在T6和cGMP中分别孵育30分钟、2小时和6小时的样本中的精子对卵子进行显微注射，受精率分别为36%、34%、29%和43%。这些受精率与这些处理后鉴定出的顶体反应精子的百分比无关。这种缺乏相关性的现象可以解释为，实际用于显微注射的顶体反应精子比例增加，特别是在T6中孵育30分钟和2小时的样本中。在未受精的卵子透明带下方可同时鉴定出顶体完整和顶体反应的精子。受精卵在体外以很高的比率发育到囊胚阶段。
