在HEK293细胞中表达的由α1β2γ2S和α1β2亚基组成的大鼠重组GABA(A)受体的脱敏特性。
Desensitization characteristics of rat recombinant GABA(A) receptors consisting of alpha1beta2gamma2S and alpha1beta2 subunits expressed in HEK293 cells.
作者信息
Krampfl K, Bufler J, Lepier A, Dudel J, Adelsberger H
机构信息
Department of Neurology of the Medical Department, Medizinische Hochschule Hannover, Germany.
出版信息
Neurosci Lett. 2000 Jan 7;278(1-2):21-4. doi: 10.1016/s0304-3940(99)00888-5.
Desensitization kinetics of rat recombinant typeA GABAergic receptors consisting of the subunits alpha1beta2gamma2S or alpha1beta2 was investigated on application of 10-0.001 mM GABA to whole cell patches using a piezo driven liquid filament switch for fast application and deapplication. At high GABA concentrations desensitization was triphasic showing increasing time constants and a decreasing extent of desensitization on lowering the GABA concentration. Below agonist concentrations of 1 mM for the trimeric receptor and 0.1 mM for the dimeric one desensitization was biphasic switching to monophasic kinetics at GABA concentrations < or = 0.01 mM for the alpha1beta2gamma2S-type and < or = 0.003 mM for the alpha1beta2-type, respectively. Comparison with former studies performed with GABAergic receptors consisting of different subunits revealed differences in the desensitization kinetics.
使用压电驱动的液体细丝开关进行快速施加和去除，在向全细胞膜片施加10 - 0.001 mM GABA的情况下，研究了由α1β2γ2S或α1β2亚基组成的大鼠重组A型GABA能受体的脱敏动力学。在高GABA浓度下，脱敏呈三相，随着GABA浓度降低，时间常数增加，脱敏程度降低。对于三聚体受体，激动剂浓度低于1 mM，对于二聚体受体，激动剂浓度低于0.1 mM时，脱敏呈双相，对于α1β2γ2S型，在GABA浓度≤0.01 mM时，对于α1β2型，在GABA浓度≤0.003 mM时，分别转变为单相动力学。与以前对由不同亚基组成的GABA能受体进行的研究相比，发现脱敏动力学存在差异。
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