Zhdan N S, Kuznetsova I L, Vlasov A V, Sil'nikov V N, Zenkova M A, Vlasov V V
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Bioorg Khim. 1999 Oct;25(10):723-32.
A series of RNA-hydrolyzing constructions was synthesized on the basis of peptide-like molecules containing residues of L-lysine, histamine and histidine methyl ester. These were shown to hydrolyze RNA effectively at neutral pH values. The in vitro transcript of tRNA(Lys) from human mitochondria and a tRNA-like fragment of RNA of Turnip Yellow Mosaic Virus were used in the experiments. Our chemical RNases quantitatively depolymerize some definite sequences (CA > or = UA > CG >> UC, CC, or CU) in both RNA molecules under optimum conditions. Moreover, no other sites were affected and no statistical hydrolysis was observed even after prolonged RNA incubation with the compounds of this series. The depolymerization rate of the RNA substrates exhibits a complex dependence on the concentration of ions of monovalent metals and on the concentration of the artificial ribonucleases.
基于含有L-赖氨酸、组胺和组氨酸甲酯残基的肽样分子合成了一系列RNA水解结构。结果表明，这些结构在中性pH值下能有效水解RNA。实验中使用了人线粒体tRNA(Lys)的体外转录本和芜菁黄花叶病毒RNA的类tRNA片段。我们的化学核糖核酸酶在最佳条件下能使两个RNA分子中的一些特定序列（CA≥UA＞CG＞＞UC、CC或CU）定量解聚。此外，即使将RNA与该系列化合物长时间孵育，也没有其他位点受到影响，也未观察到统计性水解。RNA底物的解聚速率对单价金属离子浓度和人工核糖核酸酶浓度表现出复杂的依赖性。
