Lee Y H, Han J Y, Choi A H, Lee H S, Hur W J, Seo S Y, Han H, Kim J S, Kim H J
Hematopoietic Stem Cell Transplantation Program, Dong-A Cancer Center, Dong-A University Hospital, Pusan, Korea.
J Hematother Stem Cell Res. 1999 Dec;8(6):653-7. doi: 10.1089/152581699319821.
We assessed the changes in the activities of hemostatic variables by the storage temperature and time interval between collection and separation of cord blood (CB) and analyzed their relationship with the yield of progenitor cells during processing. Total nucleated cell (TNC) and CD34+ cell counts were significantly higher in the CB stored at ambient temperature than at 4 degrees C. The significant loss of TNC and CD34+ cells continued to 24 h after collection in CB stored at 4 degrees C, but loss of TNC began only after 24 h at ambient temperature. There were no changes in the plasma activities of antithrombin III (ATIII) and plasminogen. The activity of protein C was decreased significantly until 24 h after collection, particularly in CB stored at 4 degrees C. The activity of alpha2-antiplasmin was decreased until 24 h in CB stored at 4 degrees C and from 24 h in CB stored at ambient temperature. These data suggest that the alterations in the activities of coagulation inhibitors and fibrinolytic factors could be an important factor in coagulability, particularly in CB stored at 4 degrees C compared to ambient temperature, and also affect the yield of progenitor cells in processed CB.
我们评估了储存温度以及脐带血（CB）采集与分离之间的时间间隔对止血变量活性的影响，并分析了它们与处理过程中祖细胞产量的关系。与4℃储存的CB相比，常温储存的CB中总核细胞（TNC）和CD34+细胞计数显著更高。4℃储存的CB在采集后24小时内TNC和CD34+细胞持续显著减少，但常温储存的CB中TNC仅在24小时后开始减少。抗凝血酶III（ATIII）和纤溶酶原的血浆活性没有变化。蛋白C的活性在采集后24小时内显著降低，尤其是4℃储存的CB。α2-抗纤溶酶的活性在4℃储存的CB中在24小时内降低，在常温储存的CB中在24小时后降低。这些数据表明，凝血抑制剂和纤溶因子活性的改变可能是影响凝血性的一个重要因素，特别是与常温相比，4℃储存的CB，并且还会影响处理后CB中祖细胞的产量。
