低浓度传统冷冻保护添加剂对T4Bo噬菌体低温变性损伤的预防作用
Prevention of low temperature denaturation injury in T4Bo phage by low concentrations of traditional cryoprotective additives.
作者信息
Steele P R
出版信息
J Hyg (Lond). 1976 Jun;76(3):453-8. doi: 10.1017/s0022172400055388.
Abstract
The well known cryoprotective additives glucose, sucrose, glycerol, dimethyl sulphoxide, polyvinylpyrrolidone, dextran and ammonium acetate have been found to prevent inactivation of T4Bo phage frozen in sodium bromide solutions. Their protective effects in this experimental system could not be accounted for by a colligative mechanism. It is proposed that they may act by modifying the structure of the unfrozen aqueous phase rather than by direct interaction with the phage.
摘要
人们发现，众所周知的冷冻保护添加剂葡萄糖、蔗糖、甘油、二甲基亚砜、聚乙烯吡咯烷酮、右旋糖酐和醋酸铵能够防止在溴化钠溶液中冷冻的T4Bo噬菌体失活。在这个实验系统中，它们的保护作用无法用依数性机制来解释。有人提出，它们可能是通过改变未冻结水相的结构来起作用，而不是通过与噬菌体直接相互作用。
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Some factors affecting the viability of freeze-thawed T4 bacteriophage.一些影响冻融T4噬菌体活力的因素。
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