Tomé A R, da Silva J C, Souza A A, Mattos J P, Vale M R, Rao V S
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Gen Pharmacol. 1999 Dec;33(6):479-85. doi: 10.1016/s0306-3623(99)00046-4.
Intraperitoneal injection of pilocarpine (0.75-3.0 mg/kg) caused a dose-related seminal emission in adult male rats. The seminal emission response to 3 mg/kg of pilocarpine was greatly reduced in atropinized (5 and 10 mg/kg, SC) animals, suggesting a cholinomimetic effect. Nw-nitro-L-arginine methyl ester (5, 10, and 20 mg/kg, SC), a nitric oxide synthesis inhibitor, also inhibited the pilocarpine-induced seminal emission, which was reversed by L-arginine (600 mg/kg, SC) or by coinjection of sodium nitroprusside (0.5 mg/kg, SC). Urine analysis for levels of nitric oxide metabolites, nitrate/nitrite (NO3-/NO2-), showed marked alterations in accordance with the drug treatments. The results suggest that nitric oxide mediates the inhibitory neurotransmission responsible for seminal emission in pilocarpine stimulated rats.
腹腔注射毛果芸香碱（0.75 - 3.0毫克/千克）可使成年雄性大鼠出现剂量相关的射精。在注射了阿托品（5和10毫克/千克，皮下注射）的动物中，对3毫克/千克毛果芸香碱的射精反应大大降低，提示有拟胆碱能效应。一氧化氮合成抑制剂L - 硝基精氨酸甲酯（5、10和20毫克/千克，皮下注射）也抑制毛果芸香碱诱导的射精，L - 精氨酸（600毫克/千克，皮下注射）或联合注射硝普钠（0.5毫克/千克，皮下注射）可使其逆转。对尿液中一氧化氮代谢产物硝酸盐/亚硝酸盐（NO3-/NO2-）水平的分析显示，根据药物处理情况有明显变化。结果表明，一氧化氮介导了毛果芸香碱刺激的大鼠射精过程中的抑制性神经传递。
