Saad Wilson Abrão, Gutierrez Laura Izabel, Guarda Ismael Francisco Motta Siqueira, Camargo Luis Antonio de Arruda, dos Santos Talmir Augusto Faria Brisola, Saad William Abrão, Simões Sylvio, Guarda Renata Saad
Department of Physiology and Pathology School of Dentistry, Paulista State University, UNESP Araraquara, SP, Brazil.
Life Sci. 2004 Feb 13;74(13):1593-603. doi: 10.1016/j.lfs.2003.08.027.
Male Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg/Kg (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their supraoptic nucleus (SON). We investigated the effects of the injection into the supraoptic nucleus (SON) of FK 409, a nitric oxide donor, and NW-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor (NOS), on the salivary secretion, arterial blood pressure, sodium excretion and urinary volume induced by pilocarpine, which was injected into SON. The drugs were injected in 0.5 microl volume over 30-60 s. Controls was injected with a similar volume of 0.15 M NaCl. FK 409 and L-NAME were injected at doses of 20 microg/0.5 microl and 40 microg/0.5 microl respectively. The amount of saliva secretion was studied over a five-minute period after injection of pilocarpine into SON. Injection of pilocarpine (10, 20, 40, 80, 160 microg/microl) into SON produced a dose-dependent increase in salivary secretion. L-NAME was injected into SON prior to the injection of pilocarpine into SON, producing an increase in salivary secretion due to the effect of pilocarpine. FK 409 injected into SON attenuating the increase in salivary secretion induced by pilocarpine. Mean arterial pressure (MAP) increase after injections of pilocarpine into the SON. L-NAME injected into the SON prior to injection of pilocarpine into SON increased the MAP. FK 409 injected into the SON prior to pilocarpine attenuated the effect of pilocarpine on MAP. Pilocarpine (0.5 micromol/0.5 microl) injected into the SON induced an increase in sodium and urinary excretion. L-NAME injected prior to pilocarpine into the SON increased the urinary sodium excretion and urinary volume induced by pilocarpine. FK 409 injected prior to pilocarpine into the SON decreased the sodium excretion and urinary volume induced by pilocarpine. All these roles of pilocarpine depend on the release of nitric oxide into the SON. In summary the present results show: a) SON is involved in pilocarpine-induced salivation; b) that mechanism involves increase in MAP, sodium excretion and urinary volume.
选用体重200 - 250克的雄性霍尔茨曼大鼠，用唑拉西泮50毫克/千克（氯胺酮125.0毫克和氯氮卓125.0毫克）麻醉后，将不锈钢套管植入其视上核（SON）。我们研究了向视上核（SON）注射一氧化氮供体FK 409和一氧化氮合酶抑制剂（NOS）Nω-硝基-L-精氨酸甲酯（L-NAME），对注射到视上核（SON）的毛果芸香碱诱导的唾液分泌、动脉血压、钠排泄和尿量的影响。药物以0.5微升的体积在30 - 60秒内注射。对照组注射相同体积的0.15 M氯化钠。FK 409和L-NAME分别以20微克/0.5微升和40微克/0.5微升的剂量注射。在向视上核（SON）注射毛果芸香碱后的五分钟内研究唾液分泌量。向视上核（SON）注射毛果芸香碱（10、20、40、80、160微克/微升）可使唾液分泌呈剂量依赖性增加。在向视上核（SON）注射毛果芸香碱之前，先向视上核（SON）注射L-NAME，由于毛果芸香碱的作用，唾液分泌增加。向视上核（SON）注射FK 409可减弱毛果芸香碱诱导的唾液分泌增加。向视上核（SON）注射毛果芸香碱后平均动脉压（MAP）升高。在向视上核（SON）注射毛果芸香碱之前，先向视上核（SON）注射L-NAME可使MAP升高。在毛果芸香碱之前向视上核（SON）注射FK 409可减弱毛果芸香碱对MAP的作用。向视上核（SON）注射毛果芸香碱（0.5微摩尔/0.5微升）可诱导钠和尿排泄增加。在向视上核（SON）注射毛果芸香碱之前注射L-NAME可增加毛果芸香碱诱导的尿钠排泄和尿量。在毛果芸香碱之前向视上核（SON）注射FK 409可减少毛果芸香碱诱导的钠排泄和尿量。毛果芸香碱的所有这些作用都依赖于一氧化氮向视上核（SON）的释放。总之，目前的结果表明：a）视上核（SON）参与毛果芸香碱诱导的唾液分泌；b）该机制涉及MAP、钠排泄和尿量增加。
