Mizuno M, Shiomi Y, Minato K, Kawakami S, Ashida H, Tsuchida H
Graduate School of Science and Technology, Kobe University, Japan.
Immunopharmacology. 2000 Feb;46(2):113-21. doi: 10.1016/s0162-3109(99)00163-0.
Eight species of mushrooms were evaluated for mitogenic activity by the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method using spleen cells of C3H/HeN female mice. The hot water-soluble (HWS) fraction extracted from Sarcodon aspratus showed the highest activity. The mitogen in Sa. aspratus was isolated by Sepharose 6B and DEAE-Sepharose CL-6B column chromatography. Preliminary structural analyses indicated that the mitogen was a fucogalactan. Fucogalactan elicited the release of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in macrophages of mice in vitro. TNF-alpha production induced with 50 microg/ml of fucogalactan was significantly higher than that induced by lentinan (500 microg/ml) by approximately 4.3-fold. Also, fucogalactan showed dose dependence at concentrations from 5 to 500 microg/ml in NO production. Thus, fucogalactan does elicit the release of cytokines such as TNF-alpha and NO.
采用四氮唑盐3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)法，以C3H/HeN雌性小鼠的脾细胞为材料，对8种蘑菇的促有丝分裂活性进行了评估。从糙皮侧耳中提取的热水溶性(HWS)组分活性最高。通过琼脂糖6B和DEAE-琼脂糖CL-6B柱色谱法分离出糙皮侧耳中的促有丝分裂原。初步结构分析表明，该促有丝分裂原是一种岩藻半乳聚糖。岩藻半乳聚糖在体外可诱导小鼠巨噬细胞释放肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)。50μg/ml岩藻半乳聚糖诱导的TNF-α产量显著高于香菇多糖(500μg/ml)诱导的产量，约为4.3倍。此外，岩藻半乳聚糖在5至500μg/ml的浓度范围内对NO的产生表现出剂量依赖性。因此，岩藻半乳聚糖确实能诱导TNF-α和NO等细胞因子的释放。
