Thomas M G, Bradman N, Flinn H M
The Centre for Genetic Anthropology, The Department of Anthropology, University College London, UK.
Hum Genet. 1999 Dec;105(6):577-81. doi: 10.1007/s004399900181.
We describe an integrated approach to the determination of complex Y chromosome haplotypes that is both fast and relatively inexpensive. The method employs GeneScan technology to enable a researcher to assay repeat number variation at ten microsatellite loci and determine the status of 11 diallelic polymorphisms. The method requires only four PCRs and four GeneScan runs per sample and is relatively insensitive to sample DNA concentration.
我们描述了一种用于确定复杂Y染色体单倍型的综合方法，该方法既快速又相对便宜。该方法采用基因扫描技术，使研究人员能够检测十个微卫星位点的重复数变异，并确定11个双等位基因多态性的状态。该方法每个样本仅需进行四次聚合酶链反应（PCR）和四次基因扫描，并且对样本DNA浓度相对不敏感。
