Liu W-S, Beattie C W, Ponce de León F A
Department of Animal Science, College of Agricultural, Food, and Environmental Sciences, University of Minnesota, St. Paul, MN 55108, USA.
Cytogenet Genome Res. 2003;102(1-4):53-8. doi: 10.1159/000075725.
Thirty-eight bovine Y chromosome (BTAY) microsatellites (MS) were assessed for polymorphisms in DNA samples obtained from 17 unrelated bulls. Thirty-three of these microsatellites are new and were used for the construction of a first generation radiation hybrid map for BTAY (Liu et al., 2002). Five MS had been previously reported and were used as positive controls. Fourteen out of 38 MS were found to be polymorphic; the remaining 24 were uninformative among the animals tested. The number of hemizygous loci per MS within individual ranged from two to over 20. Seven MS presented smear- or ladder-like bands, a unique feature for Y chromosome multi-copy hemizygous MS loci. The locus length variance, within individual, ranged from 2 to 42 bp corresponding to the MS with the minimum and maximum number of loci observed, respectively. Within the 14 polymorphic MS, the five pseudoautosomal MS, on average, were more polymorphic (35.3%) than the nine Y-specific MS (19.6%). Haplotypes resulting from combinations of these polymorphic loci will provide a powerful tool for future studies on the origin of domestic cattle and the evolution of bovid species.
对从17头无亲缘关系的公牛获取的DNA样本，评估了38个牛Y染色体（BTAY）微卫星（MS）的多态性。其中33个微卫星是新的，并用于构建第一代BTAY辐射杂种图谱（Liu等人，2002年）。5个MS先前已有报道，并用作阳性对照。在测试的动物中，38个MS中有14个被发现具有多态性；其余24个无信息。个体内每个MS的半合子位点数从2到20多个不等。7个MS呈现出涂片状或梯状条带，这是Y染色体多拷贝半合子MS位点的独特特征。个体内位点长度变异范围为2至42 bp，分别对应观察到的位点数最少和最多的MS。在14个多态性MS中，5个假常染色体MS平均（35.3%）比9个Y特异性MS（19.6%）具有更高的多态性。由这些多态性位点组合产生的单倍型将为未来关于家牛起源和牛科物种进化的研究提供有力工具。
