Haake S K, Yoder S C, Attarian G, Podkaminer K
Divisions of Associated Clinical Sciences, UCLA School of Dentistry, Los Angeles, California 90095-1668, USA.
J Bacteriol. 2000 Feb;182(4):1176-80. doi: 10.1128/JB.182.4.1176-1180.2000.
Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.
对具核梭杆菌的三个天然质粒进行了表征,包括对一个质粒pFN1的DNA序列分析。用pFN1构建了一种能够转化大肠杆菌和具核梭杆菌ATCC 10953的穿梭质粒pHS17。pHS17在具核梭杆菌转化子中稳定维持,转化效率的差异表明具核梭杆菌中存在限制修饰系统。