Bradshaw D J, Marsh P D, Watson G K, Allison C
Centre for Applied Microbiology & Research, Salisbury SP4 0JG, United Kingdom.
Infect Immun. 1998 Oct;66(10):4729-32. doi: 10.1128/IAI.66.10.4729-4732.1998.
Coaggregation is a well-characterized phenomenon by which specific pairs of oral bacteria interact physically. The aim of this study was to examine the patterns of coaggregation between obligately anaerobic and oxygen-tolerant species that coexist in a model oral microbial community. Obligate anaerobes other than Fusobacterium nucleatum coaggregated only poorly with oxygen-tolerant species. In contrast, F. nucleatum was able to coaggregate not only with both oxygen-tolerant and other obligately anaerobic species but also with otherwise-noncoaggregating obligate anaerobe-oxygen-tolerant species pairs. The effects of the presence or absence of F. nucleatum on anaerobe survival in both the biofilm and planktonic phases of a complex community of oral bacteria grown in an aerated (gas phase, 200 ml of 5% CO2 in air x min-1) chemostat system were then investigated. In the presence of F. nucleatum, anaerobes persisted in high numbers (>10(7) x ml-1 in the planktonic phase and >10(7) x cm-2 in 4-day biofilms). In an equivalent culture in the absence of F. nucleatum, the numbers of black-pigmented anaerobes (Porphyromonas gingivalis and Prevotella nigrescens) were significantly reduced (P </= 0.001) in both the planktonic phase and in 4-day biofilms, while the numbers of facultatively anaerobic bacteria increased in these communities. Coaggregation-mediated interactions between F. nucleatum and other species facilitated the survival of obligate anaerobes in aerated environments.
共聚是一种已得到充分表征的现象，通过这种现象特定的口腔细菌对会发生物理相互作用。本研究的目的是检查在一个模拟口腔微生物群落中共存的专性厌氧菌和耐氧菌之间的共聚模式。除具核梭杆菌外的专性厌氧菌与耐氧菌的共聚能力很差。相比之下，具核梭杆菌不仅能够与耐氧菌和其他专性厌氧菌共聚，还能使原本不能共聚的专性厌氧菌-耐氧菌对发生共聚。然后研究了在曝气（气相，200 ml含5%二氧化碳的空气/min）恒化器系统中生长的复杂口腔细菌群落的生物膜和浮游相中具核梭杆菌的有无对厌氧菌存活的影响。在有具核梭杆菌存在的情况下，厌氧菌大量存活（浮游相中>10⁷/ml，4天生物膜中>10⁷/cm²）。在无具核梭杆菌的同等培养物中，在浮游相和4天生物膜中，产黑色色素的厌氧菌（牙龈卟啉单胞菌和变黑普雷沃菌）数量均显著减少（P≤0.001），而这些群落中兼性厌氧菌数量增加。具核梭杆菌与其他菌种之间由共聚介导的相互作用促进了专性厌氧菌在曝气环境中的存活。
