Department of Environmental Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
Appl Environ Microbiol. 1986 Oct;52(4):672-6. doi: 10.1128/aem.52.4.672-676.1986.
The assimilation and respiration of glucose by attached and free-living Pseudomonas fluorescens were compared. The attachment surfaces were polyvinylidene fluoride, polyethylene, and glass. Specific uptake of [C]glucose was determined after bacterial biomass was measured by (i) microscopic counts or (ii) prelabeling of cells by providing [H]leucine as substrate, followed by dual-labeling scintillation counting. The glucose concentration was 1.4, 3.5, 5.5, 7.6, or 9.7 muM. Glucose assimilation by cells which became detached from the surfaces during incubation with glucose was also measured after the detached cells were collected by filtration. The composition of the substratum had no effect on the amount of glucose assimilated by attached cells. Glucose assimilation by attached cells exceeded that by free-living cells by a factor of between 2 and 5 or more, and respiration of glucose by surface-associated cells was greater than that by free-living bacteria. Glucose assimilation by detached cells was greater than that by attached bacteria. Measurements of biomass by microscopic counts gave more consistent results that those obtained with dual-labeling, but in general, results obtained by both methods were corroborative.
附着和自由生活的荧光假单胞菌对葡萄糖的同化和呼吸作用进行了比较。附着表面为聚偏二氟乙烯、聚乙烯和玻璃。通过以下两种方法之一测定细菌生物量后，确定了[C]葡萄糖的特定摄取量：（i）显微镜计数，或（ii）通过提供[H]亮氨酸作为底物对细胞进行预标记，然后进行双重标记闪烁计数。葡萄糖浓度为 1.4、3.5、5.5、7.6 或 9.7 μM。在与葡萄糖孵育过程中从表面上脱落的细胞的葡萄糖同化作用也在通过过滤收集脱落细胞后进行了测量。基质的组成对附着细胞同化的葡萄糖量没有影响。附着细胞同化的葡萄糖量是游离细胞的 2 到 5 倍或更多，并且与游离细菌相比，表面相关细胞的葡萄糖呼吸作用更大。脱落细胞的葡萄糖同化作用大于附着细菌。通过显微镜计数测量生物量的结果比通过双重标记获得的结果更一致，但总体而言，两种方法的结果均相互证实。
