Lachmi B E, Kääriäinen L
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1936-40. doi: 10.1073/pnas.73.6.1936.
Four nonstructural proteins with apparent molecular weights of 70,000 (ns-70), 86,000 (ns-86), 78,000 (ns-78), and 60,000 (ns-60) were translated in cells infected with Semliki Forest virus ts-1 mutant and maintained at the restrictive temperature. After synchronization of the initiation of protein synthesis these proteins were synthesized in the above order, suggesting that they are translated as a polyprotein starting from one initiation site. Two short-lived intermediates with apparent molecular weights of 155,000 and 135,000 were regularly detected. The former is presumably the precursor of proteins ns-70 and ns-86 and the latter of ns-78 and ns-60. The sequence of the structural proteins in their polyprotein was confirmed to be capsid-envelope E-2 (and E-3)-envelope E-1, beginning from the NH2-terminal end. We conclude that in Semliki-Forest-virus-infected cells two polyproteins are synthesized, one for the structural (130,000 daltons), the other for the nonstructural proteins (close to 300,000 daltons).
在感染了Semliki森林病毒ts - 1突变体并维持在限制温度的细胞中，翻译出了四种非结构蛋白，其表观分子量分别为70,000（ns - 70）、86,000（ns - 86）、78,000（ns - 78）和60,000（ns - 60）。在蛋白质合成起始同步化后，这些蛋白按上述顺序合成，这表明它们是从一个起始位点开始作为多聚蛋白进行翻译的。经常检测到两种表观分子量分别为155,000和135,000的短命中间体。前者大概是蛋白ns - 70和ns - 86的前体，后者是ns - 78和ns - 60的前体。从NH2末端开始，其多聚蛋白中结构蛋白的顺序被确认为衣壳 - 包膜E - 2（和E - 3） - 包膜E - 1。我们得出结论，在感染Semliki森林病毒的细胞中合成了两种多聚蛋白，一种用于结构蛋白（130,000道尔顿），另一种用于非结构蛋白（接近300,000道尔顿）。
