从乳腺癌患者中分离出对与乳腺癌及其他恶性疾病相关抗原具有特异性的抗体。
Isolation from patients with breast cancer of antibodies specific for antigens associated with breast cancer and other malignant diseases.
作者信息
Gorsky Y, Vanky F, Sulitzeanu D
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2101-5. doi: 10.1073/pnas.73.6.2101.
Antibodies were specifically purified, with the use of pleural fluid antigens entrapped in a polyacrylamide gel as immunoadsorbent, from a pool of sera from patients with breast cancer and from the pleural effusion of an individual patient. The purified antibodies were radioiodinated and tested for capacity to bind to the pleural fluid adsorbent. Binding of the radiolabeled antibodies was inhibited by many of the sera from women with breast cancer to a much greater extent than by sera of healthy women. With purified antibodies originating from the serum, 52% of the breast cancer sera and 16% of sera from patients with other malignancies were more inhibitory than were 95% of the sera of healthy women. In the tests with antibodies eluted from pleural fluid, 24% of the breast cancer sera and 25% of the sera from patients with other neoplastic diseases were more inhibitory than were 95% of the normal sera. We concluded that sera of patients with breast cancer may contain antibodies against antigens associated with breast cancer as well as additional antibodies against antigens that are also found in patients with other neoplastic diseases or in normal individuals. Individual breast cancer sera may inhibit binding of the labeled antibodies against breast cancer owing to their content of antibodies, antigen(s), or both.
利用包埋在聚丙烯酰胺凝胶中的胸水抗原作为免疫吸附剂，从一组乳腺癌患者的血清以及一名个体患者的胸水中特异性纯化抗体。将纯化后的抗体进行放射性碘化，并检测其与胸水吸附剂结合的能力。与健康女性的血清相比，许多乳腺癌女性患者的血清对放射性标记抗体的结合抑制作用要大得多。对于源自血清的纯化抗体，52%的乳腺癌血清和16%的其他恶性肿瘤患者血清的抑制作用比95%的健康女性血清更强。在用从胸水中洗脱的抗体进行的测试中，24%的乳腺癌血清和25%的其他肿瘤疾病患者血清的抑制作用比95%的正常血清更强。我们得出结论，乳腺癌患者的血清可能含有针对与乳腺癌相关抗原的抗体，以及针对在其他肿瘤疾病患者或正常个体中也存在的抗原的额外抗体。个体乳腺癌血清可能因其抗体、抗原或两者的存在而抑制标记抗体与乳腺癌的结合。
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