Fuĭć A, Mijić A
Institut za medicinska istrazivanja i medicinu rada, Zagreb.
Arh Hig Rada Toksikol. 1999 Sep;50(3):299-306.
Working and living environment today is an unpredictable mixture of different chemical substances. Some of these chemicals are mutagenic carcinogens and others are aneugenic carcinogens. An additional burden to the human genome is the exposure to several kinds of radiations. Among a dozen genotoxicological methods in use in vitro and in vivo, the micronucleus assay has found application in the basic research, clinical research, and pharmacological studies. The advantage of in vivo and in vitro micronuclues assays is in the fact that they provide data on both clastogenic and aneugenic action of agents. Although several years behind the in vitro technique, the in vivo micronucleus assay has swiftly developed into a significant source of data in acute and chronic genotoxic investigations, as it does not require cell culture.
当今的工作和生活环境是不同化学物质的不可预测的混合体。其中一些化学物质是诱变致癌物，而其他的则是致染色体非整倍性致癌物。人类基因组的另一个负担是暴露于几种辐射中。在体内和体外使用的十几种遗传毒理学方法中，微核试验已在基础研究、临床研究和药理学研究中得到应用。体内和体外微核试验的优势在于它们能提供有关试剂的断裂剂作用和致染色体非整倍性作用的数据。尽管体内微核试验比体外技术落后几年，但它已迅速发展成为急性和慢性遗传毒性研究中重要的数据来源，因为它不需要细胞培养。
