Salo H M, Jokinen E I, Markkula S E, Aaltonen T M
Department of Biological and Environmental Science, University of Jyväskylä, Finland.
Photochem Photobiol. 2000 Jan;71(1):65-70. doi: 10.1562/0031-8655(2000)071<0065:UBIMTI>2.0.CO;2.
The effects of a single dose of ultraviolet B (UVB) radiation (0.4 J/cm2) on immunological functions by blood leukocytes and on hematological parameters was studied in roach (Rutilus rutilus), a teleostean fish. The respiratory burst of phorbol 12-myristate 13-acetate stimulated whole blood phagocytes increased significantly after UVB irradiation but spontaneous cytotoxicity of blood leukocytes toward 51chromium-labeled K562 target cells was not markedly altered. Differential cell counting revealed that UVB exposure significantly increased the proportion of granulocytes and significantly decreased the proportion of lymphocytes in the peripheral blood, whereas hematocrit and the total number of white and red blood cells were unchanged. Plasma cortisol concentration increased in UVB-exposed fish. Severe handling stress caused similar, although not as potent, effects on the measured parameters of fish blood as UVB irradiation. These observations suggest that in fish UVB brings about a stress response, which may account for the observed alterations in the immune parameters and leukocyte composition of blood. Exposure of fish to strong visible light induced no alterations in immunological or hematological parameters, making it unlikely that ultraviolet radiation mediates its effects through visual perception.
在硬骨鱼鲤（Rutilus rutilus）中研究了单次剂量的紫外线B（UVB）辐射（0.4 J/cm²）对血液白细胞免疫功能和血液学参数的影响。佛波酯12 -肉豆蔻酸酯13 -乙酸酯刺激的全血吞噬细胞的呼吸爆发在UVB照射后显著增加，但血液白细胞对51铬标记的K562靶细胞的自发细胞毒性没有明显改变。细胞分类计数显示，UVB暴露显著增加了外周血中粒细胞的比例，显著降低了淋巴细胞的比例，而血细胞比容以及白细胞和红细胞的总数没有变化。UVB照射的鱼血浆皮质醇浓度升高。严重的处理应激对鱼血液的测量参数产生了类似但不太显著的影响，与UVB照射的影响相似。这些观察结果表明，在鱼类中UVB会引发应激反应，这可能解释了观察到的免疫参数和血液白细胞组成的变化。将鱼暴露于强光下不会引起免疫或血液学参数的改变，这使得紫外线辐射不太可能通过视觉感知来介导其作用。
