Wagner W P, Helmig D, Fall R
Department of Chemistry and Biochemistry, and Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado 80309-0215, USA.
J Nat Prod. 2000 Jan;63(1):37-40. doi: 10.1021/np990286p.
Isoprene (2-methyl-1,3-butadiene), an abundant natural product of unknown function in plants, has recently been found to be one of the major volatiles formed by Bacillus subtilis. To understand the metabolic origins of isoprene in B. subtilis, we used (13)C- and (2)H-labeling methods with GC-MS analysis of released isoprene. The results indicate that, in this bacterium, isoprene is not formed by the mevalonate pathway or from catabolism of leucine, but, as in plant systems, it is a product of the methylerythritol phosphate pathway of isoprenoid synthesis. This work supports the idea that B. subtilis could be used as a microbial model for studying the biochemistry of isoprene formation.
异戊二烯（2-甲基-1,3-丁二烯）是植物中一种功能未知的丰富天然产物，最近被发现是枯草芽孢杆菌形成的主要挥发性物质之一。为了了解枯草芽孢杆菌中异戊二烯的代谢起源，我们采用了（13）C和（2）H标记方法，并通过气相色谱-质谱联用仪对释放的异戊二烯进行分析。结果表明，在这种细菌中，异戊二烯不是由甲羟戊酸途径或亮氨酸分解代谢形成的，而是与植物系统一样，是类异戊二烯合成的甲基赤藓糖醇磷酸途径的产物。这项工作支持了枯草芽孢杆菌可作为研究异戊二烯形成生物化学的微生物模型的观点。
