具有增强固氮能力的巴西固氮螺菌Tn5突变体的特性：ORF280对nifH表达的影响。
Characterization of an Azospirillum brasilense Tn5 mutant with enhanced N(2) fixation: the effect of ORF280 on nifH expression.
作者信息
Revers L F, Passaglia L M, Marchal K, Frazzon J, Blaha C G, Vanderleyden J, Schrank I S
机构信息
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
FEMS Microbiol Lett. 2000 Feb 1;183(1):23-9. doi: 10.1111/j.1574-6968.2000.tb08928.x.
Disruption of an open reading frame (ORF) of 840 bp (280 amino acids; ORF280) in an Azospirillum brasilense Tn5 mutant resulted in a pleiotrophic phenotype. Besides an enhanced N(2)-fixing capacity and altered expression pattern of a nifH-gusA fusion, growth on the charged polar amino acids glutamate and arginine was severely affected. ORF280, similar to previously identified ORFs present in Bradyrhizobium japonicum (ORF277), Paracoccus denitrificans (ORF278) and Rhodobacter capsulatus (ORF277), exhibits in its C-terminus a significant similarity with the recently defined family of universal stress proteins.
巴西固氮螺菌Tn5突变体中一个840 bp（280个氨基酸；ORF280）的开放阅读框（ORF）被破坏，导致了多效性表型。除了固氮能力增强和nifH - gusA融合基因的表达模式改变外，在带电荷的极性氨基酸谷氨酸和精氨酸上的生长也受到严重影响。ORF280与先前在日本慢生根瘤菌（ORF277）、反硝化副球菌（ORF278）和荚膜红细菌（ORF277）中鉴定出的ORF相似，在其C末端与最近定义的普遍应激蛋白家族具有显著相似性。
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