Ogikubo Y, Norimatsu M, Kojima A, Sasaki Y, Tamura Y
National Veterinary Assay Laboratory, Tokyo, Japan.
J Vet Med Sci. 1999 Dec;61(12):1265-9. doi: 10.1292/jvms.61.1265.
Lipopolysaccharides (LPSs) were purified from Actinobacillus pleuropneumoniae serotype 2, Bordetella bronchiseptica and Haemophilus parasuis serotype 5, which were used for vaccine production in Japan, by the phenol-water procedure. In SDS-PAGE analysis, A. pleuropneumoniae LPS, as well as Escherichia coli LPS, demonstrated a typical ladder profile of a smooth-type LPS. On the other hand, B. bronchiseptica and H. parasuis LPSs lacked the ladder profiles. It was found that the biological activity of these LPSs was comparable to those of E. coli LPS in terms of activation of the clotting enzyme of Limulus amoebocyte lysate, mitogenic activity of mouse spleen cells, stimulation of TNF-alpha and nitric oxide production, but IL-6 production could hardly be observed in any LPS.
通过苯酚-水法从胸膜肺炎放线杆菌2型、支气管败血波氏杆菌和副猪嗜血杆菌5型中纯化脂多糖（LPSs），这些菌株在日本用于疫苗生产。在SDS-PAGE分析中，胸膜肺炎放线杆菌LPS以及大肠杆菌LPS呈现出典型的光滑型LPS阶梯状图谱。另一方面，支气管败血波氏杆菌和副猪嗜血杆菌LPS缺乏阶梯状图谱。研究发现，就激活鲎试剂凝血酶、小鼠脾细胞的促有丝分裂活性、刺激TNF-α和一氧化氮产生而言，这些LPS的生物活性与大肠杆菌LPS相当，但在任何LPS中几乎都观察不到IL-6的产生。
