Haga Y, Ogino S, Ohashi S, Ajito T, Hashimoto K, Sawada T
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd., Fukushima, Japan.
J Vet Med Sci. 1997 Feb;59(2):115-20. doi: 10.1292/jvms.59.115.
Protective efficacy of Actinobacillus pleuropneumoniae extracellular hemolytic toxins, RTX-toxin I (Apx I) and Apx II, was evaluated in pigs. The hemolysins were purified from culture supernatant of A. pleuropneumoniae strain HA-337, serotype 1 by immunoaffinity chromatography using a monoclonal antibody specific for Apx I or Apx II as ligand. Four pigs were vaccinated with the purified hemolysins absorbed on aluminum phosphate gel adjuvant. Four pigs of a control group were given placebo. Hemolysin-neutralizing antibodies were detected only in vaccinated group after booster injection. One of four control pigs died following an aerosol challenge with the homologous strain, and three surviving pigs developed serious clinical signs of pneumonia and had extensive lung lesions. In contrast, there was no mortality in vaccinated group. Only transient hyperthermia was observed in two vaccinated pigs after challenge. A necropsy, two vaccinated pigs had slight localized pulmonary lesions, though the remaining two had no lung lesions at all. These results indicate that the hemolysin vaccine made of Apx I and Apx II has good protective activity against swine pleuropneumonia caused by A. pleuropneumoniae serotype.
在猪身上评估了胸膜肺炎放线杆菌细胞外溶血毒素RTX毒素I（Apx I）和Apx II的保护效力。使用针对Apx I或Apx II的单克隆抗体作为配体，通过免疫亲和色谱法从1型胸膜肺炎放线杆菌HA - 337菌株的培养上清液中纯化溶血素。四只猪接种了吸附在磷酸铝凝胶佐剂上的纯化溶血素。对照组的四只猪给予安慰剂。加强注射后仅在接种组中检测到溶血素中和抗体。四只对照猪中有一只在同源菌株气溶胶攻击后死亡，三只存活猪出现严重的肺炎临床症状且肺部有广泛病变。相比之下，接种组没有死亡。攻击后，两只接种猪仅出现短暂发热。尸检时，两只接种猪有轻微的局部肺部病变，而其余两只根本没有肺部病变。这些结果表明，由Apx I和Apx II制成的溶血素疫苗对胸膜肺炎放线杆菌血清型引起的猪胸膜肺炎具有良好的保护活性。
