Zubkov M V, Sleigh M A
Division of Biodiversity and Ecology, School of Biological Sciences, University of Southampton, UK.
J Eukaryot Microbiol. 2000 Jan-Feb;47(1):62-9. doi: 10.1111/j.1550-7408.2000.tb00012.x.
Bacteria were deposited in tubes as compact pellets by centrifuging suspensions of cultured Vibrio at stationary phase. Numbers and protein biomass of flagellates added to these tubes and of the Vibrio, were followed and compared with the growth of the same and other protists on identical, uncentrifuged Vibrio. The flagellates Bodo saliens and Caecitellus parvulus, which could not be seen to multiply in tubes of suspended bacteria, grazed deposited bacteria actively as did the more versatile flagellate Cafeteria roenbergensis. The growth of these flagellates and their consumption of deposited bacteria were very similar to those of the flagellate Pteridomonas danica or the ciliate Uronema marinum fed with suspended bacteria, although deposit-feeders grew more slowly. Gross growth efficiencies (30-60%) of deposit-feeding flagellates were similar to those of the suspension-feeding protists. Caecitellus consumed 55 Vibrio to produce one flagellate, while 4,500 Vibrio were consumed to produce one Uronema. Surface-feeding flagellates are shown to be efficient bacterivores, capable of restricting the numbers of bacteria deposited on surfaces just as other protozoa control numbers of suspended bacteria.
通过离心培养至稳定期的弧菌悬浮液，将细菌以紧密沉淀的形式沉积在试管中。记录添加到这些试管中的鞭毛虫以及弧菌的数量和蛋白质生物量，并与相同的和其他原生生物在相同的未离心弧菌上的生长情况进行比较。在悬浮细菌试管中看不到繁殖的盐沼波豆虫和微小盲眼虫，与更具通用性的鲁氏海生伪角毛虫一样，能积极地捕食沉积的细菌。这些鞭毛虫的生长及其对沉积细菌的消耗，与以悬浮细菌为食的丹尼卡羽纹单胞虫或海洋尾丝虫非常相似，尽管沉积取食者生长得更慢。沉积取食鞭毛虫的总生长效率（30% - 60%）与悬浮取食原生生物的相似。微小盲眼虫消耗55个弧菌产生一个鞭毛虫，而产生一个海洋尾丝虫则消耗4500个弧菌。表面取食鞭毛虫被证明是高效的食细菌者，能够像其他原生动物控制悬浮细菌数量一样，限制沉积在表面的细菌数量。
