Laboratory of Aquatic Microbial Ecology, Department of Ecology and Environmental Sciences, Faculty of Science, Palacky University, Šlechtitelů 11, 783 71, Olomouc, Czech Republic.
Folia Microbiol (Praha). 2013 May;58(3):235-43. doi: 10.1007/s12223-012-0201-y. Epub 2012 Nov 7.
The phylogenetic composition, bacterial biomass, and biovolume of both planktonic and biofilm communities were studied in a low-order Bystřice stream near Olomouc City, in the Czech Republic. The aim of the study was to compare the microbial communities colonizing different biofilm substrata (stream aggregates, stream sediment, underwater tree roots, stream stones, and aquatic macrophytes) to those of free-living bacteria. The phylogenetic composition was analyzed using fluorescence in situ hybridization for main phylogenetic groups. All phylogenetic groups studied were detected in all sample types. The stream stone was the substratum where nearly all phylogenetic groups were the most abundant, while the lowest proportion to the DAPI-stained cells was found for free-living bacteria. The probe specific for the domain Bacteria detected 20.6 to 45.8 % of DAPI-stained cells while the probe specific for the domain Archaea detected 4.3 to 17.9 %. The most abundant group of Proteobacteria was Alphaproteobacteria with a mean of 14.2 %, and the least abundant was Betaproteobacteria with a mean of 11.4 %. The average value of the Cytophaga-Flavobacteria group was 10.5 %. Total cell numbers and bacterial biomass were highest in sediment and root biofilm. The value of cell biovolume was highest in stone biofilm and lowest in sediment. Overall, this study revealed relevant differences in phylogenetic composition, bacterial biomass, and biovolume between different stream biofilms and free-living bacteria.
本研究旨在比较不同生物膜基质（溪流聚集体、溪流沉积物、水下树根、溪流石块和水生植物）上定殖的微生物群落与自由生活细菌的微生物群落，研究了捷克奥洛穆茨市附近低阶比斯特里采溪的浮游生物和生物膜群落的系统发育组成、细菌生物量和生物体积。使用荧光原位杂交技术分析了主要系统发育群的系统发育组成。在所有样本类型中都检测到了所有研究的系统发育群。在所有基质中，溪流石块上的所有系统发育群都最丰富，而自由生活细菌的比例最低。针对细菌域的探针检测到了占 DAPI 染色细胞的 20.6%至 45.8%，而针对古菌域的探针检测到了 4.3%至 17.9%。变形菌门中最丰富的是α变形菌纲，平均占 14.2%，最不丰富的是β变形菌纲，平均占 11.4%。噬细胞-黄杆菌组的平均值为 10.5%。沉积物和根生物膜中的总细胞数和细菌生物量最高。石块生物膜中的细胞生物体积最高，而沉积物中的细胞生物体积最低。总体而言，本研究揭示了不同溪流生物膜和自由生活细菌在系统发育组成、细菌生物量和生物体积方面的相关差异。
