Cole N, Bao S, Thakur A, Willcox M, Husband A J
Cooperative Research Centre for Eye Research and Technology, School of Optometry, University of New South Wales, Sydney, New South Wales, Australia.
Immunol Cell Biol. 2000 Feb;78(1):1-4. doi: 10.1046/j.1440-1711.2000.00860.x.
Pseudomonas aeruginosa can cause ulcerative bacterial keratitis. A feature of keratitis is the rapid infiltration of the avascular corneal stroma by neutrophils. KC is a potent neutrophil chemokine. The present study used a mouse model of ocular infection to assess the relationship between KC and inflammation in the cornea in response to challenge with a strain of P. aeruginosa causing keratitis. Low levels of KC mRNA and protein were detected by in situ hybridization and ELISA, respectively, in unchallenged corneas. Dramatically increased numbers of KC mRNA+ cells were present in P. aeruginosa strain 6294-challenged corneas. Expression of KC mRNA was found to be up-regulated in the corneal epithelium in response to wounding alone. The KC mRNA+ cells were located in the epithelium and corresponding to infiltrating neutrophils cells in the stroma. Quantification of KC protein at different time points showed peak levels at 8 h of bacterial challenge. These results suggest that KC may be involved with the regulation of leucocyte infiltration early during bacterial keratitis.
铜绿假单胞菌可引起溃疡性细菌性角膜炎。角膜炎的一个特征是中性粒细胞迅速浸润无血管的角膜基质。KC是一种有效的中性粒细胞趋化因子。本研究使用眼部感染小鼠模型，以评估KC与角膜炎症之间的关系，该炎症是由一株引起角膜炎的铜绿假单胞菌激发所致。分别通过原位杂交和酶联免疫吸附测定法在未受感染的角膜中检测到低水平的KC信使核糖核酸和蛋白质。在受到铜绿假单胞菌6294菌株攻击的角膜中，KC信使核糖核酸阳性细胞数量显著增加。发现仅因创伤，角膜上皮中的KC信使核糖核酸表达就会上调。KC信使核糖核酸阳性细胞位于上皮中，与基质中浸润的中性粒细胞相对应。对不同时间点的KC蛋白进行定量分析显示，在细菌攻击8小时时达到峰值水平。这些结果表明，KC可能参与细菌性角膜炎早期白细胞浸润的调节。
