Cole Nerida, Hume Emma, Khan Shamila, Madigan Michele, Husband Alan J, Garthwaite Linda, Willcox Mark
School of Optometry and Vision Science, The University of New South Wales, Australia.
Immunol Cell Biol. 2005 Jun;83(3):301-6. doi: 10.1111/j.1440-1711.2005.01324.x.
Pseudomonas aeruginosa keratitis is one of the most destructive diseases of the cornea. The host response to this infection is critical to the outcome, and is regulated by cytokines produced in the ocular tissue. In this study, we assessed the relative contribution of the cytokines produced in the cornea to the inflammatory response of the whole eye to gain a better understanding of the inflammatory and regulatory processes in the ocular environment during localized corneal infection. C57BL/6 mice were challenged by topical application of P. aeruginosa to wounded corneas. Corneas and whole eyes were harvested 24 h post-challenge and bacterial numbers, myeloperoxidase levels and the levels of cytokines known to be important in keratitis were determined. The site of production of IL-6 and KC in the retina was determined by in situ hybridization. Before infection, 90% of macrophage inflammatory protein (MIP)-2 and approximately 80% of all IFN-gamma and IL-10 produced constitutively in the eye was found outside the cornea. Twenty-four hours after infection, bacterial numbers, levels of myeloperoxidase, and levels of MIP-2 and IL-1 were not different, whether measured in cornea or whole eye. However, expression of IL-6, KC, IFN-gamma and IL-10 was significantly greater in whole eyes than in the corneas of infected eyes. The cells expressing IL-6 and KC in the retina were identified by in situ hybridization. This study indicates that during corneal inflammation, the response of the whole eye as well as the cornea needs to be considered.
铜绿假单胞菌角膜炎是最具破坏性的角膜疾病之一。宿主对这种感染的反应对结果至关重要，且受眼组织中产生的细胞因子调节。在本研究中，我们评估了角膜中产生的细胞因子对全眼炎症反应的相对贡献，以更好地了解局部角膜感染期间眼内环境中的炎症和调节过程。通过向受伤的角膜局部应用铜绿假单胞菌对C57BL/6小鼠进行攻击。在攻击后24小时收集角膜和全眼，并测定细菌数量、髓过氧化物酶水平以及已知在角膜炎中起重要作用的细胞因子水平。通过原位杂交确定视网膜中IL-6和KC的产生部位。在感染前，发现眼内组成性产生的90%的巨噬细胞炎性蛋白（MIP）-2以及大约80%的所有干扰素-γ和IL-10在角膜外。感染后24小时，无论在角膜还是全眼中测量，细菌数量、髓过氧化物酶水平以及MIP-2和IL-1水平均无差异。然而，感染眼的全眼中IL-6、KC、干扰素-γ和IL-10的表达明显高于角膜。通过原位杂交鉴定视网膜中表达IL-6和KC的细胞。这项研究表明，在角膜炎症期间，需要考虑全眼以及角膜的反应。
