Nesje M, Røed K H, Lifjeld J T, Lindberg P, Steen O F
Division of Genetics, Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo, Norway.
Mol Ecol. 2000 Jan;9(1):53-60. doi: 10.1046/j.1365-294x.2000.00834.x.
Microsatellite DNA markers were developed from a peregrine falcon (Falco peregrinus) and genetic relationships among peregrine falcons in southern Norway were analysed using the markers. The genomic DNA library was screened for the presence of dinucleotide microsatellite repeats. Twelve loci revealed polymorphism through the initial analysis of 24 unrelated peregrine falcons, and Mendelian inheritance was confirmed in two peregrine falcon families bred in captivity. The estimated mean probability of identical genotypes in two unrelated individuals was 3 x 10-8, and the combined exclusion probability for parentage testing was 0.99 and 0.94 for one or both parents unknown, respectively. The markers were used to investigate the parentage of peregrine broods from the same nest site from different breeding seasons, and subsequently the nest-site fidelity of the breeding peregrines. High nest-site fidelity was found by studying pairwise comparisons of relatedness (rxy) estimates among chicks at six nest sites from three different breeding seasons. Cross-species amplifications showed that most loci also appeared to amplify polymorphic products in the gyrfalcon (F. rusticolus), merlin (F. columbarius), hobby (F. subbuteo) and kestrel (F. tinnunculus), demonstrating that the loci will provide powerful genetic markers in these falcons too.
从矛隼（Falco peregrinus）中开发了微卫星DNA标记，并使用这些标记分析了挪威南部矛隼之间的遗传关系。筛选基因组DNA文库以寻找二核苷酸微卫星重复序列的存在。通过对24只无亲缘关系的矛隼进行初步分析，发现12个位点具有多态性，并且在两个圈养繁殖的矛隼家族中证实了孟德尔遗传。两个无亲缘关系个体中相同基因型的估计平均概率为3×10-8，亲子鉴定中当一个或两个亲本未知时，联合排除概率分别为0.99和0.94。这些标记被用于调查来自不同繁殖季节同一巢穴的矛隼雏鸟的亲本关系，随后用于研究繁殖矛隼的巢穴忠诚度。通过研究来自三个不同繁殖季节的六个巢穴的雏鸟之间亲缘关系（rxy）估计值的成对比较，发现了较高的巢穴忠诚度。跨物种扩增表明，大多数位点在矛隼（F. rusticolus）、灰背隼（F. columbarius）、燕隼（F. subbuteo）和红隼（F. tinnunculus）中似乎也能扩增出多态性产物，这表明这些位点在这些隼类中也将提供强大的遗传标记。
