Agrawal S, Kandimalla E R
Hybridon, 155 Fortune Boulevard, Milford, MA 01757, USA.
Mol Med Today. 2000 Feb;6(2):72-81. doi: 10.1016/s1357-4310(99)01638-x.
Antisense oligonucleotides provide a simple and efficient approach for developing target-selective drugs because they can modulate gene expression sequence-specifically. Antisense oligonucleotides have also become efficient molecular biological tools to investigate the function of any protein in the cell. As the application of antisense oligonucleotides has expanded, multiple mechanisms of oligonucleotides have been characterized that impede their routine use. Here, we discuss different mechanisms of action of oligonucleotides and the possible ways of minimizing non-antisense-related [corrected] effects to improve their specificity.
反义寡核苷酸提供了一种开发靶向选择性药物的简单而有效的方法，因为它们可以序列特异性地调节基因表达。反义寡核苷酸也已成为研究细胞中任何蛋白质功能的有效分子生物学工具。随着反义寡核苷酸应用的扩展，已发现多种阻碍其常规使用的寡核苷酸作用机制。在此，我们讨论寡核苷酸的不同作用机制以及将非反义相关[已修正]效应降至最低以提高其特异性的可能方法。
