Jackson C L, Casanova J E
Service de Biochimie et Génétique Moléculaire, CEA/Saclay, Gif-sur-Yvette, France.
Trends Cell Biol. 2000 Feb;10(2):60-7. doi: 10.1016/s0962-8924(99)01699-2.
ARF proteins are important regulators of membrane dynamics and protein transport within the eukaryotic cell. The Sec7 domain is approximately 200 amino acids in size and stimulates guanine-nucleotide exchange on members of the ARF class of small GTPases. The members of one subclass of Sec7-domain proteins are direct targets of the secretion-inhibiting drug brefeldin A, which blocks the exchange reaction by trapping a reaction intermediate in an inactive, abortive complex. A separate subclass of Sec7-domain proteins is involved in signal transduction and possess a domain that mediates membrane binding in response to extracellular signals.
ARF蛋白是真核细胞内膜动力学和蛋白质运输的重要调节因子。Sec7结构域大小约为200个氨基酸，可刺激小GTP酶ARF家族成员上的鸟嘌呤核苷酸交换。Sec7结构域蛋白的一个亚类成员是分泌抑制药物布雷菲德菌素A的直接靶点，该药物通过将反应中间体捕获在无活性的流产复合物中来阻断交换反应。Sec7结构域蛋白的另一个亚类参与信号转导，并拥有一个响应细胞外信号介导膜结合的结构域。
