阿尔茨海默病患者脑脊液中α-和β-分泌酶切割的淀粉样前体蛋白水平。

Levels of alpha- and beta-secretase cleaved amyloid precursor protein in the cerebrospinal fluid of Alzheimer's disease patients.

作者信息

Sennvik K, Fastbom J, Blomberg M, Wahlund L O, Winblad B, Benedikz E

机构信息

Division of Geriatric Medicine, Karolinska Institute, NEUROTEC, KFC NOVUM, Huddinge, Sweden.

出版信息

Neurosci Lett. 2000 Jan 14;278(3):169-72. doi: 10.1016/s0304-3940(99)00929-5.

DOI:10.1016/s0304-3940(99)00929-5

PMID:10653020

Abstract

Alternative cleavage of the amyloid precursor protein (APP) results in generation and secretion of both soluble APP (sAPP) and beta-amyloid (Abeta). Abeta is the main component of the amyloid depositions in the brains of Alzheimer's disease (AD) patients. Using Western blotting, we compared the levels of alpha-secretase cleaved sAPP, beta-secretase cleaved sAPP and total sAPP, in cerebrospinal fluid (CSF) from 13 sporadic AD patients and 13 healthy controls. Our findings show significant amounts of beta-secretase cleaved sAPP in CSF. There was no statistically significant difference in the levels of beta-secretase cleaved sAPP between AD patients and controls. The levels of alpha-secretase cleaved sAPP and total sAPP were, however, found to be significantly lower in the AD patients than in the controls.

摘要

淀粉样前体蛋白（APP）的选择性切割导致可溶性APP（sAPP）和β-淀粉样蛋白（Aβ）的产生与分泌。Aβ是阿尔茨海默病（AD）患者大脑中淀粉样沉积物的主要成分。我们使用蛋白质免疫印迹法，比较了13例散发性AD患者和13名健康对照者脑脊液（CSF）中α-分泌酶切割的sAPP、β-分泌酶切割的sAPP以及总sAPP的水平。我们的研究结果显示，脑脊液中存在大量β-分泌酶切割的sAPP。AD患者和对照者之间β-分泌酶切割的sAPP水平无统计学显著差异。然而，发现AD患者中α-分泌酶切割的sAPP和总sAPP水平显著低于对照者。