急性病毒性肝炎患者乙肝表面抗原的检测

Detection of hepatitis b surface antigen in patients with acute viral hepatitis.

作者信息

Lehmann N I, Gust I D

出版信息

Aust N Z J Med. 1976 Feb;6(1):7-9. doi: 10.1111/j.1445-5994.1976.tb03283.x.

DOI:10.1111/j.1445-5994.1976.tb03283.x

Abstract

The relative value of solid phase radioimmunoassay and counter immunoelectrophoresis for the detection of hepatitis B surface antigen in the serum, was studied in 1401 patients with acute viral hepatitis. When specimens were obtained early in the illness, counter immunoelectrophoresis was found to be a reliable test for the diagnosis of hepatitis B, detecting 95.6% of patients found to be antigen positive by radioimmunoassay. The additional sensitivity of radioimmunoassay was of particular value for detecting antigen later in the illness and in patients with post-transfusion hepatitis in which the titre was frequently below the level detectable by counter immunoelectrophoresis. Radioimmunoassay was also of value in testing sera which had been stored for many years, detecting up to 3-3 times as many patients with hepatitis B as did counter immunoelectrophoresis.

摘要

在1401例急性病毒性肝炎患者中，研究了固相放射免疫测定法和对流免疫电泳法检测血清中乙型肝炎表面抗原的相对价值。在疾病早期采集标本时，发现对流免疫电泳是诊断乙型肝炎的可靠方法，能检测出放射免疫测定法显示抗原阳性患者中的95.6%。放射免疫测定法额外的敏感性对于在疾病后期检测抗原以及在输血后肝炎患者中检测抗原特别有价值，因为输血后肝炎患者的抗原滴度常常低于对流免疫电泳可检测的水平。放射免疫测定法在检测保存多年的血清时也有价值，检测出的乙型肝炎患者数量是对流免疫电泳法的3至3.3倍。