Zhang X Y, Kuznetsova L V, Zimmermann A, Wheatley A M
Department of Visceral and Transplantation Surgery, University of Berne, Inselspital, Switzerland.
Transplantation. 2000 Jan 15;69(1):3-10. doi: 10.1097/00007890-200001150-00002.
Partial portal vein ligation (PPVL) is an established approach in the study of prehepatic portal hypertension in animals. The effect of orthotopic liver transplants (OLT) on hemodynamics in PPVL animals has not been investigated to date. The aim of this study was to develop a model of OLT in PPVL rats and to investigate its hemodynamic consequences.
Three groups of male Lewis rats were investigated (1) control animals (n=7), (2) PPVL (n=9), and (3) PPVL/OLT (n=16). Three weeks after PPVL, 9 animals were taken for hemodynamic measurements. OLT was performed in the remaining 16 PPVL rats (PPVL/OLT), and, 4 weeks later, hemodynamic measurements were made. Blood biochemical analysis was performed at different time points in all 3 groups.
The PPVL animals presented with hyperdynamic systemic circulation, extensive collateral vascularization in the hilar region, and portal-systemic shunting (portal systemic shunting; 35.3+/-5.5%). In the PPVL/OLT group, 15 rats survived for 4 weeks (survival: 93.8%, 15 of 16). Of these PPVL/OLT rats, 3 died during the blood sampling protocol. In 3 PPVL/OLT rats, abnormal liver function and histology were found and deranged systemic and hepatic hemodynamics persisted after OLT. In the remaining 9 PPVL/OLT rats, systemic and hepatic hemodynamics had returned to normal at 4 weeks and portal systemic shunting was markedly reduced (2.5+/-0.9%). Liver function was in the normal range.
(1) The possibility of performing OLT in PPVL rats with a high rate of survival has been confirmed. (2) In the majority of cases, complete reversal of hemodynamic abnormalities in the PPVL animals occurs after OLT (3). PPVL/OLT represents a new and important model in OLT research.
部分门静脉结扎术（PPVL）是动物肝前性门静脉高压研究中的一种既定方法。迄今为止，原位肝移植（OLT）对PPVL动物血流动力学的影响尚未得到研究。本研究的目的是建立PPVL大鼠的OLT模型，并研究其血流动力学后果。
对三组雄性Lewis大鼠进行研究：（1）对照动物（n = 7），（2）PPVL组（n = 9），以及（3）PPVL/OLT组（n = 16）。PPVL术后三周，对9只动物进行血流动力学测量。对其余16只PPVL大鼠进行OLT手术（PPVL/OLT），4周后进行血流动力学测量。在所有三组的不同时间点进行血液生化分析。
PPVL动物表现为高动力性体循环、肝门区域广泛的侧支血管形成以及门体分流（门体分流率为35.3±5.5%）。在PPVL/OLT组中，15只大鼠存活了4周（存活率：93.8%，16只中的15只）。在这些PPVL/OLT大鼠中，3只在采血过程中死亡。在3只PPVL/OLT大鼠中，发现肝功能和组织学异常，OLT后系统和肝脏血流动力学紊乱持续存在。在其余9只PPVL/OLT大鼠中，4周时系统和肝脏血流动力学恢复正常，门体分流明显减少（2.5±0.9%）。肝功能在正常范围内。
（1）已证实PPVL大鼠进行OLT具有较高的存活率。（2）在大多数情况下，OLT后PPVL动物的血流动力学异常完全逆转。（3）PPVL/OLT代表了OLT研究中的一种新的重要模型。
