Sato M, Maulik G, Bagchi D, Das D K
University of Connecticut School of Medicine, Farmington, USA.
Free Radic Res. 2000 Feb;32(2):135-44. doi: 10.1080/10715760000300141.
Protykin is an all-natural, high potency standardized extract of trans-resveratrol (20%) and emodin (10%) derived from the dried rhizome of Polygonum cuspidatum. Previous studies have demonstrated free radical scavenging and anti-inflammatory activities of resveratrol. Since free radicals play a crucial role in the pathogenesis of myocardial ischemia/reperfusion injury, we examined whether Protykin could preserve the heart during ischemic arrest. Sprague-Dawley rats were divided into two groups: experimental group was gavaged Protykin (100 mg/kg body wt) dissolved in corn oil for three weeks, while the control group was gavaged corn oil alone. After three weeks, rats were sacrificed, isolated hearts perfused via working mode, were made globally ischemic for 30 min followed by 2 h of reperfusion. Left ventricular functions were continuously monitored and malonaldehyde (MDA) (presumptive marker for oxidative stress) formation were estimated. At the end of each experiment, myocardial infarct size was measured by TTC staining method. Peroxyl radical scavenging activity of Protykin was determined by examining its ability to remove peroxyl radical generated by 2,2'-azobis (2-amidinopropane) dihydrochloride, while hydroxy radical scavenging activity was tested with its ability to reduce 7-OH*-coumarin-3-carboxylic acid. The results of our study demonstrated that the Protykin group provided cardioprotection as evidenced by improved post-ischemic left ventricular functions (dp, dp/dt(max)) and aortic flow as compared to control group. This was further supported by the reduced infarct size in the Protykin group. Formation of MDA was also reduced by Protykin treatment. In vitro studies demonstrated that Protykin possessed potent peroxyl and hydroxyl radical scavenging activities. The results of this study indicate that Protykin can provide cardioprotection, presumably by virtue of its potent free radical scavenging activity.
普罗蒂金是一种纯天然、高浓度的标准化提取物，含有反式白藜芦醇（20%）和大黄素（10%），源自虎杖的干燥根茎。先前的研究已证明白藜芦醇具有清除自由基和抗炎活性。由于自由基在心肌缺血/再灌注损伤的发病机制中起关键作用，我们研究了普罗蒂金是否能在心脏缺血停搏期间保护心脏。将Sprague-Dawley大鼠分为两组：实验组灌胃溶于玉米油的普罗蒂金（100毫克/千克体重），持续三周，而对照组仅灌胃玉米油。三周后，处死大鼠，通过工作模式灌注离体心脏，使其全心缺血30分钟，随后再灌注2小时。持续监测左心室功能，并评估丙二醛（MDA）（氧化应激的推定标志物）的形成。在每个实验结束时，通过TTC染色法测量心肌梗死面积。通过检测普罗蒂金清除2,2'-偶氮二异丁脒盐酸盐产生的过氧自由基的能力来测定其过氧自由基清除活性，同时用其还原7-OH* -香豆素-3-羧酸的能力来测试其羟自由基清除活性。我们的研究结果表明，与对照组相比，普罗蒂金组提供了心脏保护，表现为缺血后左心室功能（dp、dp/dt(max)）和主动脉血流量改善。普罗蒂金组梗死面积减小进一步支持了这一点。普罗蒂金治疗也减少了MDA的形成。体外研究表明，普罗蒂金具有强大的过氧自由基和羟自由基清除活性。这项研究的结果表明，普罗蒂金可能凭借其强大的自由基清除活性提供心脏保护。
