漆酶介体系统氧化多环芳烃过程中的天然介体
Natural mediators in the oxidation of polycyclic aromatic hydrocarbons by laccase mediator systems.
作者信息
Johannes C, Majcherczyk A
机构信息
Institute of Forest Botany, University of Göttingen, 37077 Göttingen, Germany.
出版信息
Appl Environ Microbiol. 2000 Feb;66(2):524-8. doi: 10.1128/AEM.66.2.524-528.2000.
Abstract
The oxidation of polycyclic aromatic compounds was studied in systems consisting of laccase from Trametes versicolor and so-called mediator compounds. The enzymatic oxidation of acenaphthene, acenaphthylene, anthracene, and fluorene was mediated by various laccase substrates (phenols and aromatic amines) or compounds produced and secreted by white rot fungi. The best natural mediators, such as phenol, aniline, 4-hydroxybenzoic acid, and 4-hydroxybenzyl alcohol were as efficient as the previously described synthetic compounds ABTS [2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] and 1-hydroxybenzotriazole. The oxidation efficiency increased proportionally with the redox potentials of the phenolic mediators up to a maximum value of 0.9 V and decreased thereafter with redox potentials exceeding this value. Natural compounds such as methionine, cysteine, and reduced glutathione, containing sulfhydryl groups, were also active as mediator compounds.
摘要
在由云芝漆酶和所谓的介体化合物组成的体系中研究了多环芳烃的氧化反应。苊、苊烯、蒽和芴的酶促氧化由各种漆酶底物（酚类和芳香胺）或白腐真菌产生和分泌的化合物介导。最佳的天然介体，如苯酚、苯胺、4-羟基苯甲酸和4-羟基苄醇，与先前描述的合成化合物ABTS [2,2'-偶氮双-(3-乙基苯并噻唑啉-6-磺酸)] 和1-羟基苯并三唑一样有效。氧化效率随着酚类介体的氧化还原电位成比例增加，直至最大值0.9 V，此后随着氧化还原电位超过该值而降低。含有巯基的天然化合物，如蛋氨酸、半胱氨酸和还原型谷胱甘肽，作为介体化合物也具有活性。
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