Jang Y, Liu N, Liu J, Li H
Division of Molecular Biology, Shenyang Medical College, Shenyang, Liaoning, 110031 P.R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Feb;17(1):47-50.
To establish a method for quick RNA extraction from specified histological region in frozen sections so as to avoid cross RNA contamination among different types of cells/tissues and to achieve specific RT-PCR amplification.
Prepare serial frozen sections 5microm in thickness for histological and immunocytochemical examination. Based on the results, make one or two pieces of 20 microm section and select a specified region for quick RNA extraction and RT-PCR analysis.
RNA extraction can be finished within an hour and the data of RT-PCR obtained within 6 hours. Besides the high performance, this method can efficiently exclude pseudo positive or pseudo negative results caused by inter-cellular RNA contamination.
This method is an important complement to the current techniques for RNA extraction and RT-PCR.
