Daldrup-Link H E, Link T M, Rummeny E J, August C, Könemann S, Jürgens H, Heindel W
Department of Clinical Radiology, University of Münster, Münster, Germany.
Bone Marrow Transplant. 2000 Jan;25(1):71-8. doi: 10.1038/sj.bmt.1702087.
Our aim was to quantify irradiation-induced permeability alterations of the blood-bone marrow barrier (BMB) with dynamic contrast enhanced magnetic resonance imaging (MRI). The standard small molecular contrast agent, gadoterate meglumine, and a new macromolecular contrast agent, carboxymethyldextran-Gd-DOTA (CMD-Gd-DOTA), were compared. Twenty New Zealand white rabbits underwent MRI of the bone marrow before and 1-2 days after total body irradiation (TBI). Dynamic, repetitive T1-weighted MRI was performed before and after injection of either 0.05 mmol/kg BW CMD-Gd-DOTA (n = 10) or 0.5 mmol/kg BW gadoterate (n = 10). Bone marrow contrast enhancement was quantified as delta signal intensity: DeltaSI = |(SIpost - SIpre) / SIpre| * 100%. All MRI data were compared with the histopathologic BMB ultrastructure. Dynamic bone marrow DeltaSI data steadily increased after CMD-Gd-DOTA injection, while blood DeltaSI data slightly decreased. This bone marrow contrast enhancement, indicative of contrast agent extravasation, was significantly higher and prolonged in the irradiated group as compared to non-irradiated controls (P < 0.05) and corresponded to irradiation-induced alterations of the BMB ultrastructure seen on electron microscopy. By contrast, DeltaSI data of non-irradiated and irradiated marrow were not significantly different following gadoterate injection (P > 0.05). We conclude that irradiation-induced alterations in BMB permeability could be reliably assessed with dynamic MRI, using the new macromolecular contrast agent CMD-Gd-DOTA. Bone Marrow Transplantation (2000) 25, 71-78.
我们的目的是通过动态对比增强磁共振成像（MRI）定量评估辐射诱导的血骨髓屏障（BMB）通透性改变。比较了标准小分子造影剂钆喷酸葡胺和新型大分子造影剂羧甲基葡聚糖 - 钆 - 二乙三胺五乙酸（CMD - Gd - DOTA）。20只新西兰白兔在全身照射（TBI）前和照射后1 - 2天接受骨髓MRI检查。在注射0.05 mmol/kg体重的CMD - Gd - DOTA（n = 10）或0.5 mmol/kg体重的钆喷酸（n = 10）之前和之后进行动态、重复的T1加权MRI检查。骨髓对比增强定量为信号强度变化：ΔSI = |(SIpost - SIpre) / SIpre| * 100%。所有MRI数据均与组织病理学BMB超微结构进行比较。注射CMD - Gd - DOTA后，动态骨髓ΔSI数据稳步增加，而血液ΔSI数据略有下降。这种骨髓对比增强表明造影剂外渗，与未照射对照组相比，照射组的增强显著更高且持续时间更长（P < 0.05），并且与电子显微镜下所见的辐射诱导的BMB超微结构改变相对应。相比之下，注射钆喷酸后，未照射和照射骨髓的ΔSI数据无显著差异（P > 0.05）。我们得出结论，使用新型大分子造影剂CMD - Gd - DOTA，通过动态MRI可以可靠地评估辐射诱导的BMB通透性改变。《骨髓移植》（2000年）25卷，71 - 78页 。
