小鼠嗅觉上皮中增殖细胞和表皮生长因子受体的免疫组织化学定位
Immunohistochemical localization of proliferating cells and epidermal growth factor receptors in mouse olfactory epithelium.
作者信息
Ohta Y, Ichimura K
机构信息
Department of Otorhinolaryngology, Graduate School of Medicine, University of Tokyo, Japan.
出版信息
ORL J Otorhinolaryngol Relat Spec. 2000 Jan-Feb;62(1):20-5. doi: 10.1159/000027710.
We investigated age-related changes in proliferating cells and epidermal growth factor receptors (EGFRs) in mouse olfactory epithelium using an immunohistochemical method with the antiproliferating cell nuclear antigen (PCNA) antibody and the antihuman EGFRs antibody. Many PCNA-positive cells occurred in the surface and basal layers of the olfactory epithelium in the embryonal and neonatal mice. However, only some PCNA-positive cells occurred in the basal layer of adult mice, and only a few presented in the basal layer of aged mice. EGFRs were observed in all layers of the olfactory epithelium at the embryonal and neonatal stages, but were not identified in the olfactory epithelium at the adult or aged stages. We believe that a decrease in EGFRs in the olfactory epithelium induces the inhibition of cell proliferation, with the resultant atrophy of the olfactory epithelium.
我们使用抗增殖细胞核抗原（PCNA）抗体和抗人表皮生长因子受体（EGFRs）抗体的免疫组织化学方法，研究了小鼠嗅觉上皮中增殖细胞和表皮生长因子受体（EGFRs）的年龄相关变化。在胚胎期和新生期小鼠的嗅觉上皮表面和基底层出现许多PCNA阳性细胞。然而，成年小鼠的基底层仅出现一些PCNA阳性细胞，而老年小鼠的基底层仅有少数PCNA阳性细胞。在胚胎期和新生期阶段，嗅觉上皮的所有层均观察到EGFRs，但在成年期或老年期的嗅觉上皮中未发现。我们认为，嗅觉上皮中EGFRs的减少会导致细胞增殖受到抑制，从而导致嗅觉上皮萎缩。
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