Nakamura H
Department of Otorhinolaryngology, Niigata University School of Medicine, Japan.
Eur Arch Otorhinolaryngol. 1991;248(5):298-301. doi: 10.1007/BF00176759.
To examine the dividing cells in the olfactory epithelium, an experiment using a novel immunohistochemical technique with bromodeoxyuridine (BrdU) was performed. Tissue specimens were obtained from the olfactory epithelium of guinea pigs at days 7 and 21 after olfactory nerve axotomy. BrdU uptake was detected in the epithelial cell layer directly above the basal cell layer rather than in the basal cells per se. The BrdU-immunoreactive cells were found more numerously at 7 days than at 21 days after axotomy. The basal cells showed no immunoreaction to the anti-BrdU antibody on either day. The cells reacting with the anti-BrdU antibody also showed no reaction to the anti-cytokeratin antibody used to identify the basal cells. These findings indicate that the cells showing mitotic activity have characteristics different from those of basal cells, which has been considered previously to be the precursors of regenerating olfactory receptor cells.
为了检查嗅觉上皮中的分裂细胞，进行了一项使用新型溴脱氧尿苷（BrdU）免疫组织化学技术的实验。在嗅神经切断术后第7天和第21天，从豚鼠的嗅觉上皮获取组织标本。在基底细胞层正上方的上皮细胞层中检测到BrdU摄取，而不是在基底细胞本身。在轴突切断术后7天，BrdU免疫反应性细胞比21天时更多。在这两天，基底细胞对抗BrdU抗体均无免疫反应。与抗BrdU抗体反应的细胞对用于识别基底细胞的抗细胞角蛋白抗体也无反应。这些发现表明，显示有丝分裂活性的细胞具有与基底细胞不同的特征，而基底细胞以前被认为是再生嗅觉受体细胞的前体。
