使用固相微萃取（SPME）结合气相色谱-质谱法测定血浆中的美沙酮及其主要代谢物EDDP。
Use of solid-phase microextraction (SPME) for the determination of methadone and its main metabolite, EDDP, in plasma by gas chromatography-mass spectrometry.
作者信息
Bermejo A M, Seara R, dos Santos Lucas A C, Tabernero M J, Fernández P, Marsili R
机构信息
Department of Legal Medicine, Faculty of Medicine, Santiago de Compostela, Spain.
出版信息
J Anal Toxicol. 2000 Jan-Feb;24(1):66-9. doi: 10.1093/jat/24.1.66.
A simple, rapid method for the determination of methadone and its metabolite 2-ethylene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in plasma using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry is proposed. A 100-microm polydimethylsiloxane film fiber was exposed by immersion for 30 min in a diluted plasma solution (1:4 with buffer pH 9) containing both compounds and an internal standard (proadifen). Calibration curves were linear over the concentration range 50-2000 ng/mL. The analysis time was 45 min per sample. The determination of methadone and EDDP was subject to no interference. The performance of SPME was compared with that of liquid-liquid extraction, obtaining lower limits of detection for EDDP. The method using the two extraction procedures was applied to 10 plasma samples from methadone-treated patients.
提出了一种使用固相微萃取（SPME）和气相色谱-质谱联用技术测定血浆中美沙酮及其代谢物2-乙烯基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）的简单、快速方法。将一根100微米的聚二甲基硅氧烷薄膜纤维浸入含有这两种化合物和内标（普罗地芬）的稀释血浆溶液（与pH 9的缓冲液按1:4混合）中30分钟进行萃取。校准曲线在50 - 2000 ng/mL的浓度范围内呈线性。每个样品的分析时间为45分钟。美沙酮和EDDP的测定不受干扰。将SPME的性能与液-液萃取的性能进行了比较，得到了更低的EDDP检测限。使用这两种萃取程序的方法应用于10份来自接受美沙酮治疗患者的血浆样本。
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