回肠脂质输注刺激空肠载脂蛋白A-IV的合成，而不影响mRNA水平。

Ileal lipid infusion stimulates jejunal synthesis of apolipoprotein A-IV without affecting mRNA levels.

作者信息

Kalogeris T J, Painter R G, Holden V R

机构信息

Department of Surgery, Louisiana State University Medical Center, Shreveport 71130, USA.

出版信息

Proc Soc Exp Biol Med. 2000 Feb;223(2):198-202. doi: 10.1046/j.1525-1373.2000.22327.x.

DOI:10.1046/j.1525-1373.2000.22327.x

PMID:10654624

Abstract

We examined the effect of ileal infusions of lipid emulsion on mRNA levels and biosynthesis of apolipoprotein A-IV (apo A-IV) in jejunal Thiry-Vella fistulas in rats. The rats were surgically prepared with jejunal Thiry-Vella fistulas; after recovery they were deprived of food, equipped with ileal infusion cannulas, then given 8 hr ileal infusions of fatty acid/monoglyceride emulsions. Mucosal synthesis and transcript levels of apo A-IV in the Thiry-Vella loop were then measured. Lipid infusion produced a two-fold stimulation in incorporation of 3H-leucine into apo A-IV-specific protein, but had no significant effect on apo A-IV mRNA levels. These results support the hypothesis that a lipid-elicited, distal gut-derived, systemic signal stimulates the production of apo A-IV by a post-transcriptional mechanism.

摘要

我们研究了在大鼠空肠Thiry-Vella瘘管中回肠输注脂质乳剂对载脂蛋白A-IV（apo A-IV）mRNA水平及生物合成的影响。通过手术给大鼠制备空肠Thiry-Vella瘘管；恢复后，使其禁食，安装回肠输注插管，然后给予8小时的脂肪酸/甘油单酯乳剂回肠输注。随后测量Thiry-Vella肠袢中apo A-IV的黏膜合成及转录水平。脂质输注使3H-亮氨酸掺入apo A-IV特异性蛋白的量增加了两倍，但对apo A-IV mRNA水平无显著影响。这些结果支持以下假说：脂质引发的、源自远端肠道的全身信号通过转录后机制刺激apo A-IV的产生。